The Study Of Broilers Regulation Mechanism Under Heat Stress Using Proteomics And Phosphoproteomics

Heat stress is a common and key concern in the modern poultry industry and its exact molecular mechanism is not fully clear. Currently, most researchers focus on the function of individual gene or single pathway. In this study, in order to investigate the key proteins and sensitive indicators which global responses to heat stress, and to reveal the relevant regulatory networks of broilers under heat exposure, high-throughput and proteomics and phospho proteomics technologies were exploited to analyze the effects of heat stress on global protein in broiler chicken (Gallus gallus). The main results are summarized as follows:A total of 4,271 proteins were identified and used to generate a reference library for SWATH analysis. A total of 257 proteins showed differential expression between the control and heat stressed groups. Consistent results for twenty-six of those differential proteins were validated and confirmed by MRM analysis. Bioinformatics analysis suggests that the up- and down-regulation of these proteins appear involved in the following three categories of cellular pathways and metabolisms:1) inhibit the ERK signaling pathway; 2) affect broiler liver lipid and amino acid metabolism; 3) induce liver cell immune responses to adapt to the high temperatures and reduce mortality. The study reported here provides an insight into broiler self-regulation mechanisms and shed light on the improved broiler adaptability to high-temperature environments.Our study revealed the key proteins involved in heat response regulation, and the indicators to evaluate the heat response. We also uncovered the regulation networks of broiler heat response, and explored the related mechanisms. Our study provides a foundation for early warning in the environmental stress, evaluation of the degree of comfort, and development of strategies to ameliorate the negative impacts of heat on broilers production and welfare.