QTL Mapping Of Monoterpene Content In Grape Berry And Screening Of Candidate Genes Related To Regulation

Aroma is an important quality trait of grapes and wines.The genetic map is the basis of quantitative trait lociQTL maping.With the rapid development of molecular marker technology,grape has published more than 40 genetic linkage maps.In this study,we used 'Beifeng'(Neutral cultivars)as the female parent,and '3-34'(Muscat grape)as the material of 150 parents of male parent,and GBS was used to simplify the genome sequencing.The high density genetic map of 'Beifeng;and '3-34' was constructed,and the QTLs of the traits of monoterpene were selected.Combined with RNA-seq technology,some candidate genes related to the monoterpene synthesis were introduced.The main research results are as follows:1.The genetic map of male parent '3-34' and female parent Beifeng were constructed respectively.Among them,the genetic map of male parent 3-34 molecule formed 19 linkage groups,including 1921 SNP markers,the total map distance was 1696.8cM,The coverage rate was 84.3%and the average distance was 1cM.The female parent 'Beifeng' formed 19 linkage groups,including 2769 SNP markers,the total map distance was 1810.2 cM,the map coverage rate was 78.3%,the average map distance was 0.65cM.3.A total of 73 QTL loci were mapped to 17 monoterpenes and found that most of the QTL loci could control the results of multiple monoterpenes,QTLs and RNA-Seq analysis,and identified 32 candidates genes may be involved in monoterpene metabolism.4.The overexpression of transcription factor bZip61 can increase the content of carnation,geraniol and nerol,indicating that the transcription factor bZip61 may be synergistic with the regulation of monoterpene.