| Objective:In in vitro experiments, to study indole-3-carbinol (I3C) treatment of human nasopharyngeal carcinoma cell line CNE2cell proliferation, the apoptosis rate and PI3K/Akt pathways associated protein expression. In vivo, to study the PI3K/Akt pathway related protein expression and distant organ case by I3C nude mice bred. To study nasopharyngeal carcinoma cell line and its possible mechanism of I3C in vivo and in vitro experiments.Methods:The human nasopharyngeal carcinoma cell line CNE2was treated in different concentrations0μM,100μM,200μM and300μM of indole-3-carbinol. Then we detected cell proliferation after0,24,48and72h, apoptosis after48h and the levels of PI3K/Akt pathway-related proteins in vitro. The%$/%/F nude mice were divided into groups:prevention group, treatment group and control group. In vivo, the nude mice in every group were inoculated with nasopharyngeal carcinoma cells CNE2, and mice in prevention and treatment groups were given feed containing0.5%indole-3-carbinol. We investigated the tumoricidal effect of I3C in nude mice, and eight weeks later, the PI3K/Akt pathway-related proteins expressions in tumors from nude mice of each group were detected.Results:With the indole-3-carbinol concentration increased, cell proliferation decreased and apoptosis increased significantly. The levels of PI3K/Akt pathway-related proteins were decreased. In animal experiments, the prevention and treatment group developed smaller tumors, and the expression of PI3K/Akt pathway-related proteins in prevention and treatment groups PI3K/Akt pathway also reduced, compared to control group. Meanwhile, nearly no changes of heart, liver and kidney tissues in all groups were seen in HE staining.Conclusions:Indole-3-carbinol inhibited the growth of nasopharyngeal carcinoma cells and induced apoptosis effectively in vivo and in vitro. The mechanism might be that indole-3-carbinol could suppress PI3K/Akt pathway. |
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