Effect Of Aspirin On The Proliferation,Apoptosis,Migration And Invasion Of Osteosarcoma

Part 1 Effect of Aspirin on cell proliferation and apoptosis of osteosarcoma and mechanism researchObjective:To study the influence of aspirin on osteosarcoma cells growth and its possible mechanism.Methods:MG63 and U20S osteosarcoma cell line were cultivated and treated with various concentrations of aspirin.The cell proliferation was determined by MTT assay.After treatment with 100 mmol/L aspirin,cell cycle and apoptosis were detected using flow cytometry.The expression of apoptosis related proteins caspase-3 and p65 was detected by Western blotting.Results:The MG-63 and U20S cell proliferation are significantly inhibited by aspirin with concentration of 10mmol/L?50mmol/L?100mmol/Land 200mmol/L but not the concentration 0.1 and 1mmol/L.And the inhibition of 100 mmol/L aspirin has time dependence.MG-63 and U20S osteosarcoma cells cycle G2/M phase ratio is higher than the control group respectively(P<0.05)and the early apoptosis and lateapoptosis of MG-63 and U20S osteosarcoma cells increased significantly compared with control group(P<0.001).Tendency for 10,50 and 100/L caspas-3 protein expression of aspirin treatment group was obviously higher compared with the control group,and p65 protein expression were weaker compared with the control group.With increase concentration of aspirin,difference ofcaspase-3 and p65 protein expression intensity between the expriment group and control group is more and more obvious,respectively.Conclusion:Aspirin may promote apoptosis of osteosarcoma through regulating caspas-3 and p65.Part 2 Effects of aspirin on the migration and invasion of osteosarcoma cells and mechanism researchObjective:To investigate the effect of aspirin on the migration and invasion of osteosarcoma cells and its possible mechanism.Methods:U20S osteosarcoma cell line were cultivated and treated with 100mmol/L concentrations of aspirin for 6h.cell migration was detected by scratch assay and the cell invasion was detected by Transwell assay.Wnt/beta-catenin signaling pathway and MMP-9 and MMP-2 protein expression were detected by Western blotting.Results:The migration ability and invasion ability of U20S cells treated with aspirin were lower than that of control group,and the difference was statistically significant.Wnt3,-catenin,MMP-9 and MMP-2 protein expression levels in U20S osteosarcoma cellstreated with 100mmol/L aspirin were lower than those in control group.Conclusion:Aspirin may inhibit the invasion and migration of osteosarcoma by Wnt/beta-catenin signaling pathway.