The Clinical Significance And Mechanism Of Abnormal Expression Of SEPT Family Genes In Esophageal Squamous Cell Carcinoma

OBJECTIVE:To screen out subgroups of differentially expressed and clinical significance in esophageal squamous cell carcinoma of SEPT family and explore the differentially expressed genes between different expression groups of SEPT by bioinformatic methods to illustrate the possible presence of SEPT Mechanisms.METHODS:The mRNA expression data of esophageal squamous cell carcinoma were obtained from our laboratory,and the SEPT family gene was screened out in tumor tissue and normal tissue.Differentially expressed genes were divided into two groups according to their corresponding expression levels and survival analysis was conducted to study the effect of differentially expressed SEPT gene on the prognosis of esophageal squamous cell carcinoma.The SEPT family genes associated with the prognosis of esophageal squamous cell carcinoma were divided into high expression group and low expression group.The differential expression genes of different expression-level groups of SEPT were analyzed by edgeR and DESeq2 bioinformatic software.The potential function of differentially expressed genes was explored by GO(Gene Ontology)enrichment analysis and KEGG pathway analysis by the DAVID online analysis tool.RESULTS:Among the SEPT family genes,except for SEPT4,the remaining members were differentially expressed in tumor cells.Among them,the abnormal expression levels of SEPT6,SEPT9 and SEPT 12 were correlated with the overall survival of patients with esophageal squamous cell carcinoma.15995 and 18075 differentially expressed genes were identified by edgeR and DESeq2 between the high expression group and the low expression group of SEPT6 gene respectively.The results of the analysis of the high/low expression group of SEPT9 analyzed by two differential-expression-analysis software were 273 differentially expressed genes.Between the SEPT6 gene high-expression group and the low-expression group,edgeR and DESeq2 were used to identify the differentially expressed genes.The expression of 273 differentially expressed genes between SEPT9 high/low-expression groups was analyzed.It was found that the products of differentially expressed genes were more abundant in the cell membrane.The function was related to cell signaling transduction and cell-cell recognition;and involved in PI3K/Akt pathway.