Study Of Acoustic Radiation Force On The Control Of Viscoelastic Paricles And The Characterization Of Cells

In modern life,with the development of science and technology,there is an urgent need for the manipulation and capture of contactless nanoparticles,especially in cell and molecular biology.Particle manipulation plays an important role in studying the biomechanical properties of various cells and molecules.Particles can be captured,classified and assembled and targeted delivery of drugs can be achieved by using acoustic tweezers based on acoustic radiation force.Therefore,the use of acoustic radiation force to achieve contactless nanoparticles(cells)manipulation and capture has a great significance in the biomedical industryBased on the theory of acoustic scattering,the acoustic radiation forces acting on various viscoelastic particles and cells are studied in this paper.Chapter 1 briefly reviews the research background and practical application of acoustic radiation force.The research status of acoustic radiation force in recent years,especially the development of biomedical applications,is introduced.Finally,the main contents of the research work in this paper are summarized.In the second chapter,the characteristics of acoustic radiation force on multi-layer spherical particles in Gaussian standing wave field are studied based on acoustic scattering theory.Two kinds of particle models are studied in this paper:one is the double-layer solid-liquid spherical particle model composed of polymer materials for drug delivery;the other is the three-layer spherical particle model composed of viscoelastic materials for cell structural and mechanical properties.The influences of material and shell thickness on acoustic radiation force in multi-layer spherical structures are analyzed emphatically.Chapter 3 is based on the fact that when there are many particles in sound field,the scattering wave caused by one particle will also produce acoustic radiation force on other particles.Acoustic radiation forces of particles in the Gaussian wave field with multiple elastic particles are studied.The expression of acoustic radiation force of sparsely distributed particles is derived and the numerical simulations are carried out This study is helpful for the improvement of acoustic manipulation of small elastic particles.In the fourth chapter,the acoustic radiation forces and torques on a viscoelastic cylindrical particle near an impedance boundary irradiated by a plane traveling wave at arbitrary angle are studied.The acoustic radiation forces and torques of viscoelastic cylinders with different radius,located at different distances away from the impedance boundary and illuminated by a plane wave at different directions are simulated and analyzed.The analysis results can provide effective theoretical support for micromanipulation of biological particles in bounded space.In the fifth chapter,based on the different mechanical properties of cancer cells and normal cells,the mechanical deformabilities of kidney and bladder cancer cells in human urinary system are experimentally studied by using the acoustic radiation force This method can provide a rapid,efficient and simple method for the cellular diagnosis of urological diseases in the future.Chapter 6 makes a summary and prospects.In this thesis,the characteristics of acoustic radiation force on single and multiple viscoelastic particles in plane and gaussian waves fields are theoretically studied,and the mechanical deformabilities of two types of normal and cancerous cells are experimentally studied by using the technology of acoustic radiation force.This study has certain theoretical guidance for cell classification,identification and drug delivery in the field of biomedicine.