Synthesis Of Functional Nanoparticles And Their Applications In Bioimaging And Tumor Therapy

Cancer is a serious threat to human health,with millions of people losing their lives every year due to malignant tumors.At present,there are still no ways to eradicate many malignant tumors.Therefore,it is required to develop more advanced bio-imaging technology and better combined treatment to enhance the killing effect on malignant tumors.Based on cell imaging and detection,this paper designs and synthesizes different types of nanomaterials to achieve accurate imaging of cells and tumors and synergistic treatment of tumors.The carbon quantum dots were synthesized by one-step hydrothermal method at 180 ? for 6 h using citicoline and ethylenediamine as raw materials.It has good fluorescence performance and biocompatibility(hemolysis rate below 2%),and is basically non-toxic(the survival rate of human cervical cancer(Hela)cells cultured in 100?g/mL solution for 24 h is over 95%),which can be used for three-dimensional colorful imaging of Hela cells and human venous endothelial cells.In addition,it was found to have outstanding selectivity for Co2+ with a detection limit of 53 nM.Uniform black phosphorus quantum dots were prepared by an improved solvothermal method in N-methylpyrrolidone at 140 ? for 6 h.It has good fluorescence performance and good biocompatibility(hemolysis rate below 1%,clotting time remains basically unchanged),and is basically non-toxic(Hela cell survival rate More than 92%after incubation for 24 h in 0.5 mg/mL solution)can be used for fluorescence imaging of Hela cells.It also has good photothennal performance,and achieves photothermographic imaging of mouse underarm Hela tumors,which has no effect on normal tissues and organs of mice.In addition,the quantum dots have a good killing effect on Hela cells(the 808 nm laser is irradiated for 4 min at a concentration of 100 ?g/mL,and the 94%of Hela cells are dead).The core-shell Ag@Fe3O4 nanoparticles were synthesized by hydrothermal method at 180? for 12 h,and loaded 3 nm thick carbon film on the surface,and modified the amino-carboxypolyethylene glycol(NH2-PEG-COOH).Ag@Fe3O4@C-PEG-FA/DOX nanoparticles were prepared by grafting folic acid(FA)with amide bond and loading doxorubicin(DOX).The nanoparticles have a uniform particle size and a size of about 140 nm,and good photothermal properties(at a concentration of 150 ?g/mL,808 nm laser irradiation for 4 min,temperature up to 55?)and drug loading capacity(DOX loading 140 mg/g).It has good fluorescence imaging,magnetic resonance imaging and photothennographic effects,and has a good therapeutic effect on the underarm Hela tumor in mice.The tumor volume after treatment is 10%of its initial volume.The spindle-shaped a-Fe2O3 particles were synthesized by hydrothermal method at 100 ? for 72 h,and the gold nanoparticles were reduced on the surface,and then the amino-sulfonate polyethylene glycol and the amide bond were modified by gold-sulfur bond grafting.Finally,the a-Fe2O3@Au-PEG-Ce6-Gd nanoparticles were produced with Hydroquinone(Ce6),complexed with Gd3+.It has a certain photothermal capability(at a concentration of 50 ?g/mL,808 nm laser irradiation for 4 min,temperature up to 42 ?),and also has good nuclear magnetic resonance imaging,fluorescence imaging and photothermographic effects.It can effectively catalyze the decomposition of H2O2 in the tumor microenvironment and produce new ecological oxygen.It can be converted from Ce6 to singlet oxygen at 660 nm,which further enhances photodynamic therapy.The mouse underarm Hela tumor was subjected to photothermal and enhanced photodynamic synergy treatment,and the tumor volume after treatment was 8%of its initial volume.