Protein Functionalized Colloidal Crystal Hydrogel Beads And Their Biomedical Applications

Proteins are the main bearer of life activities and play an extremely important role in the growth and development of organisms.There are many kinds of proteins,and the levels of different proteins in organisms are usually dynamically balanced.When lesions occur in certain parts of the organism,they cause abnormal changes in some kinds of protein levels.Therefore,the detection of these abnormal changes of proteins can realize the early diagnosis of the diseases,and help to reduce the pain and economic burden of the patients,so that it has great application value.In addition,many life activities in organisms depend on enzyme catalysis,and most enzymes are proteins.In the events of pathological changes,the catalytic efficiency of enzymes will also be affected,resulting in further deterioration of the health of organisms.Colloidal crystal hydrogel beads are a kind of inverse opal structure hydrogel prepared with colloidal crystal beads as templates.It has the characteristics of small density,large specific surface area and internal structure.More importantly,the structure of the colloidal crystal beads is highly ordered,which can result in the photonic band gap,and so that the beads can display the corresponding structural color and be used for coding.Compared with traditional fluorescence coding,this kind of structural color coding will not be quenched,has better stability,and can be applied in biomedicine widely.In this paper,colloidal crystal hydrogel beads are used as coding microcarriers,and functionalized with proteins to develop their biological applications.The specific research contents are as follows:(1)Silica colloidal crystal beads were prepared based on microfluidic single emulsion system and used as template to prepare molecularly imprinted colloidal crystal hydrogel beads.The concentration and proportion of the crosslinker and the porogen in the hydrogel,the influence of the interfering protein and the response of different kinds of glycoproteins have been explored,and the results showed that the beads could detect the imprinted glycoproteins by high specificity and high sensitivity.To further investigate the detection of the beads,they were used in the analysis of the actual samples.(2)By using the silica colloidal crystal beads as templates,acrylamide as monomer and methylene diacrylamide as crosslinker,the inverse opal colloidal crystal beads were prepared.The hydrogel beads can be hydrolyzed under alkaline conditions,and be carboxylated.After activation,the enzyme can be immobilized on the beads by chemical crosslinking.It was found that the enzyme immobilized hydrogel beads have good performance in efficiency,stability and reproducibility when used in biocatalysis,which proved the feasibility of the hydrogel beads can be used as enzyme carriers.(3)The enzyme immobilized hydrogel beads were dispersed in the sodium carboxymethylcellulose solution as the internal phase,and the sodium alginate solution was used as the external phase,and the enzyme cascade reaction microcapsules were prepared by the microfluidic electrospray device.The hydrogel beads with different colors were immobilized with different kind of enzymes,and these enzymes can catalyze the substrates in cascade.Thus,the microcapsules could be used for the simulation of the function of cells or organelles,which provides a new way for the construction,research and application of organ chips.