Study On The Fabrication Of Potassium Sodium Niobite Piezoelectric Materials And Its Effect Against Cancer Cells Viability

Bone tumor is one of the most invasive cancer for humans.Moreover,bone fracture happens due to bone tumor or the surgical removal.Besides,after operation,radiotherapy and chemotherapy are applied for further treatment.Therefore,we aimed at the development of bone implant with anti-tumor effect for the use of bone fracture healing after surgical removal of tumor.In this work,potassium sodium niobite(KNN)was obtained as the research subject.The surface electric property,the piezoelectric property and the ferroelectric photovoltaic property of KNN was studied and applied for the anti-cancer characterization.1.The anti-cancer effect of the surface electric property of KNN.KNN ceramics were obtained via solid-state method.Polarization was applied and the KNN ceramics gained piezoelectric property.According to the Kelvin probe force microscopy(KPFM),the positively-poled side of KNN piezoceramics owned relatively high surface potential when compared with the negatively-poled side and unpoled side.What's more,according to the in vitro cell experiment,the positively-poled side showed anti-cancer effect whiled mild anti-cancer effect was found in negatively-poled side.The cell membrane destruction and depolarization of cancer cells was observed after the incubation on the positively-poled side of KNN piezoceramics.In a conclusion,the surface potential of KNN piezoceramics varied the cancer cell viability while higher surface potential showed anti-cancer effect.2.The anti-cancer effect of the piezoelectric property of KNN.Dynamic mechanical analyzer and picoammeters were used simultaneously for the electric potential and current signal detection of KNN piezoceramics under mechanical loading.The result showed the piezoresponse of KNN piezoceramics.Self-made mechanical loading device was obtained for the cell incubation on KNN piezoceramic with in situ mechanical loading.The cell experiment result showed that poled KNN with piezoresponse ability owned anti-cancer effect while unpoled KNN without piezoresponse barely killed cancer cells.In a conclusion,the piezoelectric property of KNN ceramics under mechanical loading could lower the cancer cell viability.3.The anti-cancer effect of ferroelectric photovoltaic property of KNN.KNN nanoparticles were obtained via solid-state method and high energy ball milling method.The anti-cancer effect of KNN nanoparticles with different concentration and different visible light irradiation condition was characterized.The in vitro cell experiment showed that the ferroelectric photovoltaic treatment was efficient and selective.The ferroelectric photovoltaic effect lowered the cancer cells viability while did not harm the normal bone cells.Moreover,cell apoptosis,overproduction of ROS and mitochondrion membrane depolarization were observed.In a conclusion,ferroelectric photovoltaic property of KNN provided the non-invasive method for cancer treatment.4.Built-in electric field with nano-scale distinction on the surface of KNN piezoceramics for cell behaviors regulation.Nanostructured polypyrrole(PPy)coatings were deposited on the KNN piezoceramics via electro-chemical method.PPy nanoarrays with good conductive property were observed via KPFM.The KNN-PPy composites owned piezoresponse ability still and the surface electric property mimicked the collagen fibrils on natural bone tissues.According to the in vitro cell experiment,the composites owned acceptable biocompatibility and could promote the osteogenic differentiation.