Fabrication Of Smart Responsive Nano-Capsule Based On Upconversion Nanomaterials

Nanotheranostic agent have been emerged as powerful nanomedicine platforms that could contribute to the conflict against many life-threatening illnesses.Many attempts have been made to apply in biomarker detection,mRNA detection,DNA detection and so on,ground on upconversion nanomaterials combining upconversion luminescent imaging and drug release,due to their unique photo-physical properties,deep tissue penetration,excellent light stability and low toxic to biological tissue.However,many of those upconversion drug delivery systems often have low drug rate,complex structures,large size,time-consuming multistep preparation and bad repetitiveness.To solve those problems,we worked three parts.Such as:?1?We built a novel drug delivery system using mesoporous inner-empty upconversion microspheres carrying drugs with ZnO quantum dots?UCMPS@ZnO?acted as a‘door keeper'for pH-triggered drug release.Compared to other upconversion drug delivery systems,it is smarter,simpler,more efficient and more biocompatibity.In particular,the UCMPS@ZnO microspheres shows low cytotoxicity,simple production and have high drug loading rate?15%?.Promisingly,mice treatment experiment demonstrated that the multifunctional nanoparticles have efficient inhibition of tumor growth after 14-day treatment.It makes UCMPS@DOX-ZnO microspheres a promising candidate theranostic in cancer treatment and cilinical trials.?2?We constructed a novel microenvironment-sensitive nanotheranostic agent,Gd₂O₃:Yb³⁺/Er³⁺@DOX-ZnO,based on mesoporous inner-empty upconversion microspheres.It served as an MRI and UCL probe for locating tumors in vivo.The tumour-specific acidic environment induces dissolution of the ZnO nanoparticles coated onto the microspheres,triggering the release of doxorubicin hydrochloride?DOX?at the tumor site.More importantly,by establishing colorectal cancer mice model treated with nanotheranostic agent for two weeks,it shows that tumor growth can be inhibited significantly.The highlight of this work is that nanotheranostic agent effectively cures colorectal cancer,which could be used in getting good theranostic effects on the other cancers.?3?Silica coated-NaYF₄ upconversion nanomaterials?NaYF₄@SiO₂?of various shapes-nanorods,nanodumbbell and nanowire-were synthesized through controlling the ratio of NaYF₄ nanorods to silica.The nanodumbbell and nanowire morphologies showed much higher fluorescence efficiency than that of nanorod.These upconversion nanoparticles?UCNPs?exhibited a high signal-to-noise ratio,effectively circumvent luminescence quenching and excellent photo-stability under980 nm excitation.In particular,the as-prepared nanodumbbells were successfully applied to rapid latent fingerprint imaging,showing advantages over commercial fluorescence reagents.Such nanoparticles with further modification are expected to have a great potential in forensic and biological sciences.