Construction Of Multi-functional Membranes With Inorganic Microspheres/Polymer And Their Basic Study On Application

Detection chips are widely used in clinical laboratory science,drug synthesis screening,environmental monitoring and protection,health quarantine,judicial authentication and biological reagent detection,which has advantages of easy operation,rapid reaction,non-post treatment and non-environment pollution.Clinical laboratory market has expanded rapidly with the promotion of Hierarchical Medical System,which shows a broad market prospect of clinical detection chips.Clinical detection chips which have peculiarity of easy-take and rapid reaction can realize POCT?point-of-care testing?with miniaturization instrument.Clinical detection chips with multi-functional membrane structure have advantages of convenience but also good accuracy and high precision.Unfortunately,limited by the serum disperse material,there are not a series of clinical detection chips with multi-functional membranes.Having broken the limitations of polymer as the main material of inorganic/polymer composite membrane which leads to a low reflectivity,multi-functional membranes of dispersion,filter and reflection were constructed with cellulose acetate as adhesive and controllable synthesized high-reflective microspheres such as TiO₂,BaSO₄,and BaTiO₃ as the main material.Then the multi-functional membranes were used in clinical detection chips.?1?Precipitation method was used to controllable synthesize TiO₂ microspheres which have the characteristics of 2³?m size,narrow distribution of partial size,monodisperse,and morphological regularity.Controllable synthesis conditions such as:reactant mass ratio,reactant content,solvent volume ratio,dispersant concentration,reaction time,reaction temperature,and calcinate time all coordinated morphology,partial size and distribution of partial size.TiO₂/CA multi-functional membrane constructed with controllable synthesized TO₂ microspheres and cellulose acetate was smooth and thermal stable.The light reflectivity of the TiO₂/CA multi-functional membrane in the 460⁸00 nm range was higher than 90.7%.The area SD of uniform colorful spots formed by serum diffused in the TiO₂/CA multi-functional membrane and reacted in the reagent membrane was 0.04.The multi-functional membrane could be used in clinical detection chips.?2?Complexation method was used to controllable synthesize BaSO₄ microspheres which have the characteristics of 1.5?m mean size,narrow distribution of partial size,monodisperse,and morphological regularity.Controllable synthesis conditions such as:reactant mass ratio,reactant content,reaction time,reaction temperature,pH,stirring rate and ripening time all coordinated morphology,partial size and distribution of partial size.The yield of BaSO₄microspheres was efficiently increased to 74.15%by optimizing reaction concentration,mass ratio and reaction time.The yield could further increase to 87.88%when pH was adjusted once again to 7.0 but the decrease the diameter homogeneousness.Furthermore,BaSO₄/CA multi-functional membrane constructed with controllable synthesized BaSO₄ microspheres and cellulose acetates showed high light reflectivity,92.0%,in the range 400⁷50 nm.The area SD of uniform colorful spots was 0.02.The multi-functional membrane could be used in clinical detection chips.?3?Precipitation method was used to controllable synthesize BaTiO₃ sub-microspheres which have the characteristics of 0.5?m size,narrow distribution of partial size,monodisperse,and morphological regularity.Controllable synthesis conditions such as:reactant mass ratio,reactant content,reaction time,stirring rate,reaction temperature,and calcinate time all coordinated morphology,partial size and distribution of partial size.BaTiO₃/CA multi-functional membrane constructed with controllable synthesized BaTiO₃sub-microspheres and cellulose acetate showed a very high light reflectivity,97.5%,in the range of 460⁸00 nm.Serum diffused in the BaTiO₃/CA multi-functional membrane reacted in the reagent membrane and then formed uniform colorful spots.The area SD of uniform colorful spots SD was 0.055.The multi-functional membrane could be used in clinical detection chips.?4?GLU clinical detection chips were constructed with TiO₂/CA,BaSO₄/CA,and BaTiO₃/CA multi-functional membranes,and their standard curves,linear range,repeatability,and accuracy were detected.GLU clinical detection chip constructed with BaTiO₃/CA has the widest linear range which ranged from 1.11 to 16.65 mmol/L.The standard curve equation was y=0.6089-0.184 ln x,r²=0.9982.SD and CV of 3 GLU clinical detection chips constructed with TiO₂/CA,BaSO₄/CA,and BaTiO₃/CA were 0.24,0.20,0.32 and 2.8%?2.4%,3.9%,respectively,which is lower than limiting value.Accuracy of 3 GLU clinical detection chips were within target value range.