| Bactrocera dorsalis Hendel belongs to family of Tephritidae in Diptera.It is an invasive alien pest and a key prevention target in China.B.dorsalis has become a dangerous pest damaging fruit and vegetable,and its prevention and control strategy has received widespread attention.Existing method,such as chemical pesticides may cause environmental pollution.The method of artificial bagging wastes time and energy.Therefore,it is urgent to explore more simple and effective methods of prevention in order to achieve green and sustainable field management of B.dorsalis.This study explored genetic control methods and techniques from the perspective of flight capacity and wing development.The main results are as follows.(1)This study compares the flight capabilities of two important economic fruit flies,Bactrocera dorsalis and Bactrocera correcta.B.dorsalis flies farther and lasts longer than B.correcta in 12 hours,while the dorsal longitudinal muscles of B.dorsalis are more developed.The establishment of transcriptome libraries and the analysis of differential expression genes shows that B.dorsalis accounts for a greater advantage in the genes annotated to the flight capacity.(2)In this study,the structure of larval wings is observed by dissection,antibody staining and frozen sections.It is found that the wings of B.dorsalis are cystic and composed of peripodial epithelium cell and columnar cells.During development,the peripodial epithelium cells partially degraded and are fused with the epidermis,turning the wings from the body to the outside and completing folding to form a double layer,and the winged cells form the adult wings after further stretching.Real-time quantitative PCR is used to analyze the expression patterns of the wing development genes screened from the transcriptome library,and it is found that the expression of the wing development genes is significantly up-regulated in the pupal stage.At the same time,the expression of the different wing-development genes in the wing disc of B.dorsalis is respectively revealed by the in situ hybridization,showing the regions where each gene functions.(3)This study establishes a superior method and system for RNA interference(RNAi)in B.dorsalis,which can successfully synthesize high-quality dsRNA.The nanomaterial carrier introduces dsRNA to cells by feeding,the expression of 10 related wing development genes(wingless,vestigial,cubitus interruptus,apterous,decapentaplegic,hedgehog,optomotor blind,ultrabithorax,engrailed,epidermal growth factor receptor)are inhibited,and the individuals with impaired wing are formed.The detection of RNAi efficiency shows that dsRNA plays a role in individual of B.dorsalis.(4)In this study,RNAi is initially applied to the control of B.dorsalis.Wingless(wg)is selected for field simulation based on phenotype,mortality and malformation rate.The artificial bait containing dswg preparation is used to attract females to lay eggs.The statistical data of the adult number of the next generation and the rate of fruit damage indicate that the population of the offspring fed with the RNA preparation are significantly reduced,and the rate of fruit failure is significantly reduced.This study provides theoretical support for the application of pest genetic control,and provides new ideas and methods for solving the current problem of the severe spread and damage of B.dorsalis.It is significance for protecting the safety of agricultural and forestry production,and promoting the trade of fruits and vegetables. |
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