The Role Of Non-coding RNAs In Brucella Interacted With Host Cells

The Non-coding RNAs(ncRNAs)are key regulators of bacterial metabolism,virulence and adaptation to environmental stresses,and play important roles in gene expression regulations which are triggered by environmental cues and stresses.At present,the research of ncRNAs mainly focuses on several kinds of model organisms,but it remains poorly understood about bacterial ncRNAs.We performed genome-wide ncRNAs and functional genomics analysis in Brucella.Through small RNA sequencing,we obtained the genome-wide nc RNAs(0 to 50 nt)of Brucella.Through the RNA sequencing,we obtained the genome-wide ncRNAs(50 to 150 nt)of Brucella.Through strand-specific RNA deep-sequencing approach,we obtained the genome-wide ncRNAs(100 to 500 nt)of Brucella.In total,3878 ncRNAs were identified.These ncRNAs differ in their expression levels and strand and chromosomal distributions.In this study,by using a combined bioinformatic and experimental approach,two novel ncRNAs(Bru-DH783 and Bru-DH997)were identified in Brucella.Bru-DH783 and Bru-DH997 were further examined and found to relate the intracellular survival of Brucella.Bru-DH783 and Bru-DH997 were highly induced during infection of host macrophages.The ncRNAs of Brucella were associated with ABC transporters,protein export,Two-component system,Ⅲ and IV secretion system.Here,we demonstrate the existence of microRNA-size,small RNAs(msRNAs)in Brucella.The ncRNAs of host cells infected with Brucella were analyzed.By analyzing the ncRNAs of host cells infected with Brucella,the role of ncRNAs in Brucella interacted with host cells were studied.Here,we obtained the comprehensive analysis of miRNA expression profiles in Brucella infected macrophage cells.Differentially expressed miRNAs were identified by using RT-qPCR.The GO enrichment analysis suggests that the target genes of these differentially expressed miRNAs are involved in lysosome,endocytosis and vitamin D-dependent antimicrobial pathway.Here,we obtained the comprehensive analysis of mi RNA and LncRNA expression profiles in Brucella infected the trophoblast cell line HTR-8/SVneo.Differentially expressed miRNAs were identified by using RT-qPCR.The GO enrichment analysis suggests that the target genes of these differentially expressed miRNAs are involved in MAPK,P53,WNT,Gap junction,endocytosis,adherens junction and T cell receptor signaling pathway.The GO enrichment analysis suggests that the target genes of these differentially expressed LncRNAs are involved in P53,apoptosis,gap junction,adherens junction,proteasome and lysosome.Our results provide the first direct evidence that LncRNAs can regulate Brucella infection.Brucella non coding RNA can regulate host cell function.It plays an important role in the interaction between Brucella and host cells.By RNAomic analysis,our results provide that Brucella exists a large number of high abundance of ncRNAs,which can regulate host cell function.The ncRNAs of Brucella are involved in lysosome and autophagy signaling pathway,which are closely related to the immune escape of Brucella.The ncRNAs mediated by Brucella play an important role in the interaction between Brucella and host cells.MiR-21 and miR-146 a promote the intracellular survival and colonization of Brucella,which can affect pathogenic infection by regulating host metabolism and gut microbiota.In this study,we analyzed the ncRNAs of Brucella and host cells infected with Brucella.The role of non coding RNA in the interaction between Brucella and host cells was studied.MiR-21 can be used as a target for regulating intracellular infection.It lays the foundation for the development of new drug targets and vaccines.