Long Non-coding RNA SNHG7 Competing Interacted With Mir-193b Regulating The Expression Of FAIM2 To Affect The Progression Of Lung Cancer

lung cancer is one of common maligancy and the leading cause of mortality worldwide,which greatly threats the security and life quality of human beings.With the development of medical technology,although the clinical treatment of lung cancer has largely improved,there is still large room to improve the treatment of lung cancer in clinic,and the 5-year survival of patients is less than 20%.Recent studies have proved tha non-coding RNAs have play critical regualtory roles in the proression of cancers.miR-193b is a common studies small non-coding RNA in tumors,which has been explored to closely related with the proliferation,apoptosis,migration,and invasion of tumor cells.However,studies of miR-193 is still simple in cancer.Using the public bioinformatics database,this study had explored that miR-193b could interact with FAIM2 and lncRNA SNHG7,indicating that these three RNAs might be interacted with each other.RT-PCR assay was used to detect the tumor and adjacent tissue of lung cancer patients and lung cancer cell lines and normal epthelial cells,and identified that expression level of miR-193b was significantly decreased in tumor tissue and cancer cell lines compared with the adjacent tissues or normal epthelial cells,while the expression levels of FAIM2 and IncRNA SNHG7 were significantly increased in tumor tissue cancer cell lines compared with the adjacent tissues or normal epthelial cells.Luciferase reporter assay and overexpression of miR-193b could interacted with FAIM2 to inhibit the expression levels of FAIM2 in lung cancer cell lines.Overexpression of FAIM2 could significantly promote the proliferation,migration,and invasion of A549 cells but evidently inhibited the apoptosis of A549 cells;while overexpression of miR-193b coud significantly inhibit the effects of FAIM2 on the proliferation,apoptosis,migration,and invasion of A549 cells;namely,promote the apoptosis of A549 cells,inhibit the proliferation,migration,and invasion of A549 cells.Overexpression of miR-193b could significantly enhance the interaction with IncRNA SNHG7 in A549 cells.Overexpression of IncRNA SNHG7 could significantly promote the expression level of FAIM2,and decrease expression level of miR-193b.Overexpression of miR-192b could significantly inhibit the proliferation of A549 cells but promote the apoptosis of A549 cells;while overexpression of IncRNA SNHG7 could significantly inhibit the effects of miR-193b on the proliferation and apoptosis of A549 cells.Overexpression of miR-192b could significantly inhibit the migration and invasion of A549 cells,while overexpression of IncRNA SNHG7 could significantly inhibit the effects of miR-193b on the migraiton and invasion of A549 cells.An in vivo transplatation tumor assay had confirmed that downregulating the expression of IncRNA SNHG7 could significantly inhibit the growth volume of tumor,but overexpression of IncRNA SNHG7 could evidently promote the growht volume of tumor.RT-qPCR in tumor tissues had detected that downregulating the expression of IncRNA SNHG7 could obviously reduce the expression level of FAIM2 but promote the expression of miR-193b;however,upregulating the expression of lncRNA SNHG7 could significantly decrease the expression of miR-193b but increase the expression of FAIM2.All of the above findings have indicated that miR-193b could interact with FAIM2 and lncRNA SNHG7.LncRNA SNHG7 could serve as a ceRNA for miR-193b interacting with miR-193b to upregulating the expression level of FAIM2,so that to promote the proliferation,migration,and invasion of lung cancer and inhibit the apoptosis of lung cancer.Therefore,it might be important to reveal the regulatory relationship among IncRNA SNHG7,miR-193b,and FAIM2,so that could improve the diagnosis and treatment of lung cancer in clinic and promote the exploration of new targte drugs for lung cancer.