Expression Of SYK In Retinoblastoma/Pseudoretinoblastoma And The Role Of SYK On Apoptosis Of Retinoblastoma Tumor Cells

Objective 1.To detect the expression of SYK in the tissue of 62 retinoblastomas,9 pseudoretinoblastomas and 6 nonneoplastic lesions in retina;2;To analysis the correlation between SYK expression and nercosis or high risk factors in retinoblastoma;3.To detect the expression of SYK in human retinoblastoma tumor cells Y-79 and WERI-RB-2;4.To analyse the effect of 8406(SYK inhibitor)on apoptosis of Y-79 tumor cells and WERI-RB-2 tumor cells;5.To analyse the effect of R406(SYK inhibitor)on an Y-79 tumor cells or WERI-RB-2 tumor cells transplanted BALB/c mouse mode;Methods 1.Inmmuchemical stain SYK expression in 62 retinoblastomas,9 pseudoretinoblastomas and 6 nonneoplastic Lesions in retina;2.Analyze the correlation between SYK expression and nercosis or high risk factors of retinoblastoma by Spearman rank method;3.Immunofluorescent stain SYK expression in Y-79 tumor cells and WERI-RB-2 tumor cells;4.Inhibit SYK activity by R406 in both Y-79 tumor cells and WERI-RB-2 tumor cells so as to study the role of SYK inhibitor in tumor cell' s activity and apoptosis as well as to measure the expression of caspase-3 in tumor cell s by flow cytometer;5.Inhibit SYK activity by R406 in Y-79 and WERI-RB-2 transplanted BALB/c mouse to study the role of SYK inhibitor in tumor growth and transplanted tumor cell' s apoptosis.Results SYK is 100%positive in the tissue of 62 retinoblastomas while is negative in 9 pseudoretinoblastomas and 6 nonneoplastic lesions of retina;2.Correlation analyse showed there's negative correlation between SYK expression and nercosis(P<0.01)but there's no correlation between SYK expression and high risk factors(P=0.126)in retinoblastoma;3.SYK is also expressed in human retinoblastoma tumor cells Y-79 and WERI-RB-2;4.SYK inhibitor R406 promotes tumor cells Y-79 and WERI-RB-2 apoptosis and increase the level of caspase-3 in tumor cells;5.There's a mass in BALB/c mouse after Y-79 tumor cells or WERI-RB-2 tumor cells transplanted.A wide rang of necrosis appeared in animals dealt with SYK inhibitor R406 while good tumor cells' viability is observed in animals didn't treated with SYK inhibitor R406.Conclusions 1.SYK is exceptionally a good maker in retinoblastoma to differentiate malignant tumors from benign neoplasm as well as to assitst in distinguishing primary lesions from metastatic lesions,there's negative correlation between SYK expression and necrosis but there's no correlation between SYK expression and high risk factors in retinoblastoma;2.SYK also is positive in human retinoblastoma tumor cells Y-79 and WERI-RB-2;3.SYK inhibitor R406 promote tumor cells Y-79 and WERI-RB-2 apoptosis and increase the level of caspase-3 in tumor cells,in addition to,8406 increase tumor cell's apoptosis in Y-79 and WERI-RB-2 transplanted BALB/c mouse,so we concluded that SYK play a promoting role in tumorigenesis in retinoblastoma.