A Study On The Role Of Long Noncoding RNA-UCA1 In Myocardial Ischemia/reperfusion Injury And Its Molecular Mechanism

Background and PurposeLong noncoding RNAs(lncRNAs)play an important role in cell physiology process.Urothelial carcinoma-antigen 1(UCA1)is a recently identified long noncoding RNA(lncRNA).However,few studies have explored its role in cardiomyocytes after focal myocardial ischemia reperfusion injury(MI/RI).MethodsRat MI/RI models were established using ligation of the Lower Anterior Descending artery(LAD).Cell apoptosis and reactive oxygen species(ROS)production in cardiac tissues were explored using immunohistochemistry and DHE staining.lncRNA expression patterns were detected using microarray and validated by qPCR.Cell viability and apoptosis were examined using MTT assay and flow cytometry.ResultsMI/RI significantly induced cell apoptosis and ROS production in the rat model.The results of microarray demonstrated the reduced expression of UCA1,which was validated by qPCR.Follow-up experiments showed that UCA1 was involved in H2O2-induced cell apoptosis.We further showed that UCA1 negatively correlated with the expression of p27.Moreover,overexpression of p27 could induce primary cardiomyocyte apoptosis.ConclusionsReduction of UCA1 levels plays a proapoptotic role in primary cardiomyocytes partially through stimulation of p27 protein expression.These results are in agreement with the observed levels of UCA1,p27 and apoptosis after MI/RI,suggesting that UCA1 might have important role MI/RI.