| Background and objective Lung cancer has become one of the major causes of malignant tumor-related deaths worldwide.At present,surgery is the main treatment method for non-small cell lung cancer,but there are still 30-70%of patients with non-small cell lung cancer arise recurrence and metastasis after surgery.At present,the ways for monitoring the recurrence and metastasis of postoperative non-small cell lung cancer patients,including imaging,serology,and pathology,all have some lag and one-sidedness.Circulating tumor cells are known as an important way for tumor recurrence and metastasis,and the count in peripheral blood is closely related prognosis of non-small cell lung cancer patients.In addition,epithelial-mesenchymal transition is currently considered to be a very important part of the process of circulating tumor cells.This study summarized and analyzed the relationship between peripheral blood circulating tumor cell,its types and clinicopathologic features in 30 patients with non-small cell lung cancer,in order to provide new ideas for the detection of tumor recurrence and metastasis.Method This study included 30 non-small cell lung cancer patients that were confirmed by histological or cytological ways in the Department of Thoracic Surgery of Peking Union Medical College Hospital between November 2016 and June 2017,and 5 ml of peripheral blood was collected within one week before surgery.We used CanPatrol technology to detect peripheral blood circulating tumor cells,which combing nanofiltration membrane filtration technology with mRNA in situ hybridization technology to collecting circulating tumor cells and different circulating tumor cell types can be identified in real time.After the detection of circulating tumor cells,the clinical and pathological features of included non-small cell lung cancer patients and detection data were analyzed.Results Among the enrolled patients,there were 18 male patients and 12 female patients with an average age of 64.5 years.Among the 30 patients,there were 15 patients were diagnosed as adenocarcinoma and 15 patients were diagnosed as squamous cell carcinoma.In this group of patients,the tumor stage was distributed from stage Ⅰ-Ⅳ,and 28 patients in the group had circulating tumor cells detected in the peripheral blood,with an average of 6.4 circulating tumor cell per patients.In this group of patients,71 epithelial circulating tumor cells were detected,97 mixed circulating tumor cells were detected,and 25 mesenchymal circulating tumor cells were detected.According to the rank correlation analysis,the positive rate of circulating tumor cells in the peripheral blood was associated with smoking history(P=0.016).The positive rate and number of circulating tumor cells in the peripheral blood were increased in patients with previous smoking,and it was confirmed that smoking was associated with lung squamous cell carcinoma(P=0.008),and the diameter of lung tumors in previously smoked patients was relatively large(P=0.024).Smoking patients had larger tumor diameters than non-smokers during surgery.In addition,the analysis between lymph node metastasis and other variables was found it is correlated with gender(P=0.044).Lymph node metastasis was more likely to occur in male patients.Conclusion The CanPatrol technology used in this experiment successfully isolated circulating tumor cells from the preoperative peripheral blood of patients with non-small cell lung cancer,and carried out synchronous typing,which the circulating tumor cells were identified as epithelial type,mixed type and mesenchymal type.Through Rank correlation analysis,the positive rate of circulating tumor cells is found related to the smoking history of patients with non-small cell lung cancer.Circulating tumor cells are more easily detected in the peripheral blood of smoking patients,and lymph node metastasis is more likely to occur in male patients.The trial has not yet found significant differences in the number and classification of circulating tumor cells in different pathological types of non-small cell lung cancer.We look forward to a larger sample size and the inclusion of follow-up data to draw more clinically meaningful conclusions related to circulating tumor cells and their classification.Background and objective At present,lung cancer has become one of the major causes of cancer-related death in the world.Studies found that the amplification of fibroblast growth factor receptor 1 existing in various solid malignant tumors.Fibroblast growth factor receptor 1 has been found to play a role in the development of non-small cell lung cancer.Its amplification expression is associated with some clinical features of non-small cell lung cancer,and often indicates a relatively poor prognosis.Now,many targeted drugs targeting non-small cell lung cancer are on the market,but the prognosis of non-small cell lung cancer is still not optimistic.Fibroblast growth factor receptor 1 is currently considered as one of the potential targets for the treatment of non-small cell lung cancer.This study collected and detected the expression of fibroblast growth factor receptor 1 in peripheral blood circulating tumor cells in 30 patients with non-small cell lung cancer,and analyzed its relationship with clinicopathological features.Method This trial included 30 patients with non-small cell lung cancer from November 2016 to June 2017 in the Department of Thoracic Surgery of Peking Union Medical College Hospital.5ml of peripheral blood was collected as a test specimen within one week before surgery.The CanPatrol technology was used during the study.This technique can detect the expression level of fibroblast growth factor receptor 1 gene in circulating tumor cells in the same time using the immunostaining technique of in situ mRNA hybridization.After the testing,the clinical and pathological data of the enrolled patients,peripheral blood circulating tumor cell test results and fibroblast growth factor receptor 1 gene expression in circulating tumor cells data were summarized.We performed a multivariate correlation analysis using rank correlation analysis and rank sum test to explore whether there was a statistically significant association between the above three factors.Results Fibroblast growth factor receptor 1 gene expression can be detected in peripheral blood circulating tumor cells in patients with non-small cell lung cancer.According to the expression level of fibroblast growth factor receptor 1 gene,it was identified as no expression,low expression,medium expression and high expression level.No expression,low expression,medium expression,and high expression levels can be detected in peripheral blood circulating tumor cells in different types of non-small cell lung cancer patients,and the above four types of expression levels in different types of circulating tumor cells can be seen.No difference was found in the expression of fibroblast growth factor receptor 1 in circulating tumor cells in different pathological types of non-small cell lung cancers by Mann-Whitney U test(P=0.806).Kruskal-Wallis test did not find any differences in the expression level of fibroblast growth factor receptor 1 in three types of circulating tumor cells(P= 0.202,P= 0.094).The rank correlation analysis also found no correlation between fibroblast growth factor receptor 1 in circulating tumor cells and sex,smoking history,and tumor staging.Conclusion The technique used in this trial can detect the expression of fibroblast growth factor receptor 1 gene in peripheral blood circulating tumor cells in patients with non-small cell lung cancer.In the test results of the patients enrolled in this trial,there was no difference in the expression of the fibroblast growth factor receptor 1 gene in peripheral blood circulating tumor cells in patients with different pathological types of non-small cell lung cancer.In addition,the test data of patients enrolled in the trial did not find any significant difference in the expression level of fibroblast growth factor receptor 1 gene expression in different types of circulating tumor cells.Constrained by the smaller sample size,we expect that the inclusion of a larger sample size will yield different results. |
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