Based On The ERK1/2 Signal Pathway, The Mechanism Of E₂/T Imbalance On The Pathogenesis Of BPH And The Intervention Effect Of Yishen Tonglong Capsule On BPH Model Rats Were Explored

Objective:To study the effects of Yishen Tonghuang Capsule on prostate tissue morphology,serum T,E₂/T ratio,receptor expression of AR,ER?and ERK1/2 related signaling pathway and apoptosis in BPH rats by E₂+T synergistic modeling method,and to explore the possible mechanism of Yishen Tonghuang Capsule on prostate hyperplasia.METHODS:64 male SD rats were divided into blank group,model group,treatment group,Yishen Tongzheng high,medium and low dose group?0.96g/Kg d,0.48g/Kg d,0.24g/Kg d?.After administration,the wet weight of prostate and the index of prostate were measured,the pathological changes of benign prostatic hyperplasia tissues stained with HE were observed,the contents of E₂ and T in serum of rats were detected by Elisa,apoptosis in paraffin sections of prostatic tissues was detected by TUNEL,and the expressions of AR,ER?,ERK1/2,c-Jun and Raf-1 in prostatic tissues of rats were detected by RT-PCR.Result:Yishen Tonghuang group could reduce the wet weight of prostate and prostate index of rats,which had significant difference compared with model group?P<0.05?;could improve the pathological changes of prostatic tissue hyperplasia;Yishen Tonghuang capsule high and middle dose group could reduce serum T and E₂levels and increase serum E₂/T,which had significant difference compared with model group?P<0.05?;The high,middle and low doses of capsules could promote the apoptosis of prostate cells in BPH model rats,which had significant difference compared with the model group?P<0.05?;the high,middle and low doses of Yishen Tonglong capsules could down-regulate the expression of AR,ER?,ERK1/2,c-jun,Raf-1 and other related genes,which had significant difference compared with the model group?P<0.05?.CONCLUSION:1.By observing the prostatic hyperplasia model induced by estrogen and androgen,we found that the model was successful.2.The occurrence of BPH may be due to E₂/T imbalance and activation of ERK1/2 signaling pathway.3.Yishen Tonghuang Capsule at high and low doses and control group Longbishu Capsule can reduce the wet weight of prostate and prostate index,improve the morphology of prostate cells in BPH rats,and have a certain therapeutic effect on prostate hyperplasia;and the curative effect of Yishen Tonghong Capsule at medium doses=control group=high doses group>low doses group of Chinese medicine;Yishen Tonghong Capsule at high and low doses can promote BPH model rats.Apoptosis of prostate cells can improve the therapeutic effect of hyperplasia and promote apoptosis:middle dose group of Yishen Tonghuang capsule=control group>high dose group>low dose group of traditional Chinese medicine.4.Yishen Tonghuang Capsule at high and medium doses can reduce serum T and E₂ levels and increase serum E₂/T,suggesting that Yishen Tonghuang Capsule may interfere with BPH by affecting the sex hormone level of experimental rats.5.Yishen Tonghuang Capsule can down-regulate the expression of AR,ER?,ERK1/2,c-jun,Raf-1 and other related genes at high,middle and low doses,which may interfere with BPH model rats by inhibiting ERK1/2 signaling pathway.6.Yishen Tonghuang Capsule is superior to high dose and low dose...