| Osteosarcoma occurs in children and adolescents in the period of bone growth.Due to its high malignancy and high disability rate,once the recommended surgical treatment is found,the current clinical routine treatment is still surgical resection and radiotherapy and chemotherapy.However,the surgery has the disadvantages of incomplete resection or excessive resection.Chemotherapy is for systemic treatment,lacking "targeting" for tumor cells,and easily causing systemic toxicity and drug resistance of tumor cells.Nano-material-based photothermal therapy A novel minimally invasive treatment technique for tumors emerges,that is,the use of synthetic nanomaterals to convert the light energy of near-infrared laser(NIR)into heat energy,thereby denaturing and necrosis of tumor cells and killing tumor cells..The key to photothermal therapy is to develop effective photothermal nanomaterials to achieve the effect of killing tumor cells without harm to surrounding tissues.In order to make photothermal materials in addition to photothermal therapy,a multi-functional nanomaterial with enhanced computerized tomography(CT)contrast is developed to provide more effective and comprehensive diagnosis and treatment of osteosarcoma.Based on the above ideas,this paper explores the preparation of two common photothermal nanomaterials,copper and bismuth,and prepares them into CuS-PVP and Bi2S3-PVP,and performs photothermal treatment,antibacterial performance and enhancement of osteosarcoma.The performance such as CT development was studied.(1)preparation of CuS-PVP,photothermal therapy and antibacterial activity in osteosarcoma Although the photothermal conversion efficiency of copper sulfide is relatively high,its water solubility is poor and its biocompatibility is poor,which greatly limits its possibility of becoming a biological agent.Therefore,we designed and developed nano biomaterial CuS-PVP.Nano material CuS was prepared by solvothennal method,and its formation mechanism and morphology were verified by powder X ray diffraction(XRD)and scanning electron microscope(TEM).The water heat method was used to modify CuS with PVP to make it good water solubility and biocompatibility,because its excellent photothennal conversion efficiency(52.10%)provided a good premise for the photothennal material to treat osteosarcoma.The hemolysis test proved that it had a very low hemolysis rate.The cell test proved that it had good in vitro killing.The function of dead osteosarcoma cells in nude mice proves that it has good function to kill osteosarcoma cells in the body.H&E staining proves the safety of the osteosarcoma cells in vivo,and the good antibacterial properties of CuS-PVP can reduce the postoperative complications while killing tumor cells.(2)preparation of Bi2S3-PVP,photothermal therapy and X ray tomography(CT)imaging in osteosarcoma.We hope to synthesize a nanoscale material that can kill tumors through light heat and also have the function of X ray tomography(CT),with a view to providing the precise treatment of osteosarcoma.Based on the above idea,we have synthesized Bi2S3 by thermal solvent method,and we have verified the formation mechanism and morphology by powder X ray diffraction(XRD)and scanning electron microscope(TEM).The PVP was also modified by hydrothermal method,and its good biocompatibility in vitro was verified by cell test and hemolysis test,and its photothermal conversion efficiency(25.42%)was measured,the possibility of killing osteosarcoma cells was found,and the effect of killing osteosarcoma cells in vitro was proved by cell test,and then through the body of nude mice.Experiments and H&E tests showed that the body could also kill osteosarcoma cells as well as good biocompatibility in the body.The injection of Bi2S3-PVP through the rat tail vein also made us get a clear image under CT. |
PDF Full Text Request