The Expression And Significance Of Fatty Acid Metabolism Enzymes In The Prefrontal Cortex And Cerebellum In Autistic Brain

(?)the Expression and Significance of Fatty Acid Metabolism Enzymes in the Whole Brain,Prefrontal Cortex And Cerebellum In C57BL/6 Mice At Different AgesObjective To investigate the expression pattern of fatty acid metabolism enzymes n the whole brain,prefrontal cortex and cerebellum in C57BL/6 mice at different ages.Methods 24 SPF class male C57 BL / 6 mice were used.Specifically,six for P0 Postnatal Day 0),six for P14(Postnatal Day 14),six for P21(Postnatal Day 21),six or P180(Postnatal Day 180).Whole brain tissue was stripped from each mouse.Three ice in each age group were used for Western Blot experiments,the other three for mmunohistochemistry.In addition,another nine C57 BL / 6 mice were used.Three for 14(Postnatal Day 14),three for P28(Postnatal Day 28),three for P42(Postnatal Day 2)respectively.Prefrontal cortex and cerebellum were stripped from each mouse for estern Blot experiments.Results(1)The protein levels of fatty acid metabolism enzymes acetyl coenzyme carboxylase(ACC),the phosphorylation of acetyl-coenzyme A carboxylase(pACC) nd fatty acid synthase(FAS)in C57BL/ 6 mice increased with age;the alterations of rotein expression levels of acetyl coenzyme A synthetase 1(AceCS1)and long-chain cyl-coenzyme A synthetase 1(ACSL1)in C57BL/ 6 mouse brains were first increased nd then decreased with age.(2)The expression levels of FAS,ACC and pACC in erebellum and prefrontal cortex of C57 BL / 6 mice are the highest at 2 weeks of age.Conclusions(1)Fatty acid synthesis in mice brain may decrease with increasing ge.(2)Fatty acid ?-oxidation increased in early brain development in B6 mice,and ay reduce in late brain development.(?)The Expression and Significance of Fatty Acid Metabolism Enzymes n The Prefrontal Cortex And Cerebellum In BTBR MiceObjective To investigate the expression difference of fatty acid metabolism nzymes in the prefrontal cortex and cerebellum in BTBR and C57BL/6 mice.Methods 18 SPF class male BTBR and C57 BL / 6 mice were used.Specifically, ine BTBR mice and nine B6 mice.The prefrontal cortex and cerebellum of mouse rains were used.Six mice in each age group were used for Western Blot expereiments, he other three for immunohistochemistry.Results Compared with wild-type C57BL/6 mice,autism model BTBR mice anifested significant increase in FAS,AceCS1,pACC and ACC protein expression in erebellum;in addition,significant upregulation of FAS,AceCS1,pACC and ACSL1 n cortex were detected in BTBR mice.Conclusions Increased fatty acid synthesis and ?-oxidation in the cerebellum and refrontal cortex in BTBR mice may be detected.(?)The Expression and Significance of Fatty Acid Metabolism Enzymes in the Prefrontal Cortex and Cerebellum in Autistic Brain SpecimensObjective To investigate the expression difference of fatty acid metabolism enzymes in the prefrontal cortex and cerebellum in the brain specimens of autistic subjects and control subjects.Methods Eight autistic brain specimens and eight control brain specimens were used.The prefrontal cortex and cerebellum of the brain specimens were detected using Western Blot method.Results Compared with the control group,children with autism experienced increased expression of ACSL1,AceCS1 and ACC in cerebellum specimens;in addition,increased expression of ACSL1 in cortex was found in cortex specimens of autistic children.Conclusions Increased fatty acid ?-oxidation in the cerebellum and prefrontal cortex in autistic subjects may be detected.