Clinical Research On The Role Of Y Chromosome Microdeletion Detection In Male Infertility Diagnosis And Treatment And The Relationship Between It And Sex Hormone

BACKGROUND:Y-chromosome microdeletions is a common cause of spermatogenesis disorders in male infertile patients.In 1976,Tiepolo et al.demonstrated for the first time that there is a close relationship between Y chromosome microdeletions and male infertility.Since then,the study on genetic predisposition of male infertility has become a hot topic of scholars,especially on the study of AZF gene,and the deletion and reproduction of AZF gene.There is no unified conclusion about the relationship between hormones.OBJECTIVE:To compare the distribution of Y chromosome microdeletions and the changes of sex hormone levels in patients with azoospermia and severe oligozoospermia infertility,and to analyze the correlation between Y chromosome microdeletions and sex hormones.Obtain a more comprehensive understanding of the clinical data of male infertility Y microdeletion,so as to provide a more comprehensive basis for the detection of Y microdeletions in clinical male infertility patients,and help to predict the prognosis of assisted reproductive technology and the choice of treatment methods for infertile patients.Avoid unnecessary medical operations and save on medical costs.METHODS:364 infertile patients were collected.Semen routinely showed azoospermia or severe oligozoospermia.The age,reproductive hormone(mainly FSH,INHB),and Y chromosome microdeletion were collected and grouped by EXCEL 2007.The clinical parameters were analyzed by SPSS21 software.The normal distribution data was expressed by X±s.using t-test to compare the mean between the two independent samples,and the ?2 test was used to compare the two groups.All tests are two-sided.P<0.05 was defined as the difference was statistically significant.RESULTS:The rate of deletion in patients with non-obstructive azoospermia was 10.3%(30/290),and the rate of deletion in patients with severe oligozoospermia was 8.1%(6/74).The levels of deletion were basically the same,no statistical difference(P>0.05).).According to age,all enrolled patients were divided into three groups:group A(18-30 years old),group B(31-40 years old),and group C(41-49 years old).There was no significant difference in the rate of Y chromosome microdeletion between the three groups(P>0.05).The regression model established by INHB and FSH is statistically significant.That is,INHB=-0.616FSH+106.467,and the regression coefficient was significant value P<0.05,which was statistically significant.The INHB values were divided into the INHB normal group and the INHB lower group.The deletion rate of Y microdeletions between the two groups was observed.The normal group deletion rate was 13.5%(27/200),and the reduced group loss rate was 3.3%(3/91).The difference between the two groups was statistically significant(P<0.05),and the statistical deletion rate was higher than that of the normal group when INHB was decreased.CONCLUTIONS:The most common type of Y chromosome microdeletion is AZFc deletion,followed by AZFb deletion and AZFa deletion;The Y chromosome microdeletion rate of patients with azoospermia and severe oligozoospermia is basically the same.The occurrence of Yq microdeletions does not increase with age;The absence of SY127 is characterized by azoospermia and severe oligozoospermia;The FSH and INHB test results are negatively correlated and are not a substitute for INHB detection.We speculate that FSH is more sensitive to testicular spermatogenesis than INHB;When INHB is lower than normal,Y chromosome microdeletion rate is increased,INHB can be used as a predictor of Y chromosome microdeletion,but due to the existence of differences,undeniable FSH Predictive effects,more clinical data needs to be further collected;We recommend that clinically infertile men with severe oligozoospermia should be advised to perform Y-chromosome microdeletion testing.