Knockdown Of MSI1 Inhibited The Proliferation Of Human Oral Squamous Cell Carcinoma By Inactivating STAT3 Signaling

ObjectiveTo clarify the molecular mechanism of MSI1(Musashi-1)in promoting the proliferation,invasion and migration of oral squamous cell carcinoma(OSCC).Materials and MethodsWestern blot assays were used to analyze the expression of physophylated STAT3 and total STAT3 in oral squamous cell carcinoma tissues and paired normal tissues.Hence,MSI1 levels in 30-paired OSCC tissues and paired adjacent healthy tissues were detected using q RT-PCR methods.To screen OSCC cell lines,select experimental cells,and detect the effects of MSI1 interference and overexpression on cell proliferation,invasion and migration.To study the expression of c-myc,cyclin D,p21,p27 and STAT3 after MSI1 interference and overexpression.To study the effects of interfering MSI1 on the growth of oral squamous cell carcinoma in nude mice.ResultsThe expression of MSI1 and phosphorylated STAT3 in OSCC was high.MSI1 promotes the proliferation,invasion and migration of OSCC cells.Interference with MSI1 decreased the expression of c-myc,cyclin D and increased the expression of p21 and p27,and also inhibited the tumor growth in animal experiments.It is also proved that there is a binding site between msi1 and c-myc.ConclusionsMSI1 promoted cell proliferation,invasion and migration partly through activation of STAT3 signaling pathway in OSCC.