The Preparation And Bioanalytic Applications Of Lanthanide Coordination/Doped Materilas

Introducing antenna molecules is the mainly method to enhance the fluorescence of lanthanide fluorescence probes.However,most high-efficiencyantenna molecules are prepared via complex organic synthesis reactions.Therefore,it is becoming a new research approach to use novel ways to increase the fluorescence of lanthanide and basing the structural properties of determinand itself toachieve specific testing purpose.In this paper,on the one hand,we took lanthanide ions as the luminescence center and introduced antenna molecules to prepare metal-organic framework material with stable structure.According to the structural properties of determinand itself,the lanthanide probe can realize the specific detection.On the other hand,without the antenna molecule,lanthanide ions were doped into carbon dots(C dots),and the C dots can increase the fluorescence of lanthanide ions sharply The materials mentioned above both can increase the fluorescence of lanthanide ions,which steadily applied in biological samples1.Detection of cyanide via extended ?-conjugation-induced fluorescence enhancement of a metal organic framework composed of terbium(?),bipyridyl and adenosine diphosphateA metal-organic framework(MOF)was designed and prepared from luminescent Tb(?),adenosine diphosphate(ADP)and bipyridyl(Bipy).Extended ?-conjugation occurs between MOF and cyanide,whicheffectively sensitizes the fluorescence of Tb(?).Therefore,we want to realize the detection of cyanide via the principle of ?-conjugation-induced fluorescence enhancement.This principle can provide high specificity,because the principle is based on the specificspace structure between the probe and determinand.The probe even can be used to detect cyanide in saliva samples with complex compositions,and the limit of detection is as low as 30 nMIts green fluorescence at 545 nm is shown to enable the fluorometric detection of cyanide ion based on the principle of ?-conjugation-induced fluorescence enhancement.The fluorescence of the probe is strongly increased by cyanide due to extended ?-conjugation between probe MOF and cyanide which sensitizes the fluorescence of Tb(?).This effect can be used to quantify cyanide at levels as low as 30 nM in aqueous solution.The method was applied to the determination of cyanide in saliva samples.Based on thecharacteristies of linear structure of cyanide,the method realizes thespecific detection of Tb-ADP-Bipy MOF to cyanide via the principle of ?-conjugation-induced fluorescence enhancement.The lack of interference by acetate and fluoride is effectively overcome2?Carbon Dots and Terbium Co-Enhanced Fluorescence of Europium Nanoparticles for Cell ImagingEu(?)and Tb(?)ions were doped into carbon dots(C dots)for the preparation of C:EuTb nanoparticles(C:EuTb NPs)via one-pot synthesis.Because of the dual energy transfer effects from C dots and Tb(?)ion to Eu(?)ion,the fluorescence of Eu(?)in C:EuTb NPs is enhanced 336.8-fold without any antenna molecules.The intense fluorescence of C:EuTb NPs is not easily quenched in aqueous solutions due to the insertion of Eu(?)ions into C dots,which reduces the quenching from water molecules,overcoming the weakness of fluorescence quenching of lanthanide materials in water.Not only in aqueous solution,solid C:EuTb NPs also present strong fluorescence because of the doped structure.C:EuTb NPs show excellent biological safety;the viability of MCF-7 is more than 90%even at a concentration of 1250 ?g·mL-1 of C:EuTb NPs.MCF-7 cells stained with C:EuTb NPs display distinct red fluorescence in the fluorescent field of microscope.C:EuTb NPs are promising to be used as a red coloring agent for biological observations3?Lanthanide doped carbon dots as a fluorescence chromaticity-based pH probeA colorimetric and fluorescent pH probe was designed by doping carbon dots(C-dots)with Eu(?),Tb(?)and 2,6-pyridinedicarboxylic acid(DPA).The resulting nanoparticles were applied as fluorescent indicators for pH values(best detected at excitation/emission wavelengths of 272/545,614 nm).The pH induced optical effects are due to pH induced variations in energy transfer.The fluorescence of the probe shows a continuous color variation,and a linear change with pH values in the range from 3.0 to 10.0 can be established by using a Commission Internationale de L,Eclairage(CIE)chromaticity diagram.This new kind of pH nanoprobe is more accurate than previously reported pH indicator probes because the pH value can be calculated by using chromaticity coordinates that only depend on the chromaticity.The pH nanoprobe was applied to visualize pH values in human breast adenocarcinoma cells(MCF-7).