Femtosecond spectroscopic study of isolated-chromophore and exciton-state photophysics in C-phycocyanin

e have employed femtosecond transient hole-burning (THB) spectroscopy to examine isolated-chromophore and coupled-chromophore photophysics of C-phycocyanin, a photosynthetic light-harvesting protein located in the rods of the phycobilisome in cyanobacteria. C-phycocyanin consists of two polypeptide subunits which covalently bind phycocyanobilin (open-chain tetrapyrrole) chromophores at three sites. With preparations of the ;In the...