Study on two methylotrophic and halophilic methanogens, Methanosarcina siciliae HI350 and Methanolobus taylorii GS-16

train HI350 was isolated from an oil well in the Gulf of Mexico. This strain was similar to Methanolobus siciliae T4/M;Degradation of dimethyl sulfide or methane thiol by strain HI350 was complete, and stoichiometric quantities of methane and hydrogen sulfide were formed. Studies of cell-free extracts suggested that enzymes for the degradation of dimethyl sulfide and methane thiol were inducible, whereas those for the degradation of methanol or trimethylamine were constitutive.;Methanolobus taylorii GS-16, a moderately halophilic and alkcaliphilic methanogen, grows over a wide pH range from 6.8 to 9.0. Cells suspended in medium with pH above 8.2 reversed their...