Methods to achieve high resolution in three-dimensional cryo-electron microscopy of macromolecules and application to the 70S Escherichia coli ribosome

Despite the progress in X-ray crystallography and NMR, there are still many macromolecular complexes whose structures cannot be determined using these two methods. Cryo-EM is a powerful tool to study those macromolecular complexes. However, the resolution of 3D reconstructions from non-crystalline cryo-EM images is only around 1/30 A...