Part I. Iron(II) and ruthenium(II) nitrosyl complexes as peptide-related scavengers and carriers of nitric oxide. Part II. [Palladium(II)(methyliminodiacetate)(peptide)] complexes as models for immobilized metal ion affinity chromatography (IMAC)

Several areas of biochemistry that are potentially amenable to advancements in transition metal chemistry have been investigated. A new synthetic methodology to make the H2edampda ligand, a two pyridyl, two-carboxylate analogue of edta 4− was achieved. The ligand edampda2− was then complexed with a variety of transition metals to examine the ligand's adaptability in allowing various coordination geometries. Coordination to iron(II) as a possible scavenger of nitric oxide received more attention. Investigations into ruthenium complexes as chemotherapeutic agents were made by investigating the coordination of [Ru(edta)] with 5′-GMP. Further studies also involved developing new peptide and iminodiacetate coordinated ruthenium nitrosyls as possible new chemotherapeutic photodynamic agents. Computer methods were also used to further investigate the coordination of five and six mer peptides to [Pd(mida)] to model immobilized metal ion affinity chromatographic technique (used in commercial ewe purification and in protein separations) to add intellectually-based choice to which amino acid peptide tags would be the preferred ones to exploit for uses in immobilized metal ion affinity chromatography (IMAC).