| In this work, new micromachined magnetic particle separators and integrated biofilters have been proposed, developed, and successfully characterized for an integrated magnetic bead-based biochemical detection system. Physical behavior of magnetic particles has been investigated to develop a dynamic monitoring method of magnetic bead separation. In addition, magnetic separation force on the magnetic beads has been simulated by analytical and numerical methods to optimize performance. Developed magnetic particle separators and biofilters have been fully characterized for both dynamic monitoring capability of magnetic bead separation and biofiltering performance.; The developed biofilters and biosensors have been integrated with a microfluidic system to realize an integrated microfluidic biochemical detection system for fast and small volume immunoassays using magnetic beads, which are used as immobilization surfaces as well as bio-molecule carriers. Magnetic bead-based immunoassay, as a typical example of biochemical detection and analysis, has been performed on the integrated microfluidic biochemical detection system that includes a surface-mountable biofilter and immunosensor. From the immunoassay performed with the biochemical detection system, 50 ng/ml of sample concentration (e.g., mouse IgG) has been successfully detected and analyzed. Total analysis time required for the full immunoassay was less than 20 minutes including sample incubation time. Sample volume used was less than 10 μl during one immunoassay. Protein sampling capability has been also demonstrated by capturing target antigens.; The methodology developed in this work can be also applied to generic bio-molecule detection and analysis systems by replacing antibody/antigen with appropriate bio receptors/reagents such as DNA fragments or oligonucleotides, which will be useful for DNA analysis and high throughput protein analysis. |
