Resonance Raman structural characterization of cyanide ligated cytochrome P450cam

Cytochromes P450 are the strongest known in vivo oxidizing agents whose essential function is the oxidative metabolism of a large variety of compounds. The essential goal of the present work is to utilize resonance Raman (RR) spectroscopy to study the active site structure of cyanide ligated cytochrome P450cam as an analogue to the ferric-superoxo P450cam, an intermediate involved in the oxidation of the substrate.;It has been recently proposed that the key factor in catalyzing the cleavage of the bound O;Careful analyses of the difference patterns obtained by subtraction of various pairs of spectra of four CN...