Kinetic isotope effects and hydrogen tunneling in the yeast triosephosphate isomerase reaction

Secondary kinetic isotope effects were measured for the base-catalyzed proton exchange reaction of acetophenone in deuterium oxide at 25;The secondary deuterium kinetic isotope effects of the deuteroxide-ion catalyzed exchange of methyl protons of acetophenone in D;The secondary kinetic isotope effects for the yeast triosephosphate isomerase reaction are normal (KIE ;The secondary H/T and D/T kinetic isotope effects for the (1(Re)-...