| Section 1: Affinity chromatography. A high-performance affinity chromatographic column containing immobilized human serum albumin (HSA) was used for the chiral separation of D- and L-tryptophan and to study the binding of these compounds to HSA. Frontal analysis was used to examine changes in the association equilibrium constant (K;Section 2: Capillary electrophoresis. The theory and mechanism of chiral separations and solute-ligand binding in capillary electrophoresis based on the use of running buffer additives were examined in this study. Human serum albumin (HSA) and ;Section 3: Dicamba degradation study. The degradation of herbicide dicamba by bacteria Pseudomonas maltophilia DI-6 strain was studied by using CE, GC/MS and FAB/MS. A simple and fast CE method was developed for the analysis of dicamba degradation sample and confirmed that 3,6-dichlorosalicylic acid (DCSA) is one of its major degradation products. Also, the present studies clearly show that in addition to the previously established demethylation pathway through DCSA, there is another degradative pathway which has the direct dehalogenation of dicamba as its first step. And three types of bacteria enzyme activities (demethylation, methylation and dehalogenation) were observed in the degradation of dicamba by P. maltophilia DI-6. |
