| In this dissertation, I report my studies on the resistance of Arabidopsis thaliana ecotype Dijon (Di-0) to turnip crinkle carmovirus (TCV), a simple, single-stranded, positive-sense, plant RNA virus. TCV efficiently replicated in protoplasts prepared from Di-0 callus culture, but it did not move long-distance in Di-0 plants (Simon et al., 1992). Cardamine chlorotic fleck virus (CCFV), the carmovirus most closely related to TCV, infected Di-0 plants systemically (Oh et al., 1995). I found that TCV with the coat protein open reading frame (ORF) from CCFV (TCV-CP;Many plant RNA viruses are associated with small subviral RNAs, including satellite (sat-) RNAs. Small subviral RNAs require a helper virus for replication and movement, and they often intensify or attenuate the symptoms caused by the helper virus. TCV is associated with sat-RNA C, a sat-RNA that normally intensifies the symptoms of the TCV-M isolate (Simon et al., 1988; Li and Simon, 1990). I will report my work on the mechanism of symptom modulation mediated by sat-RNA C in Arabidopsis plants. My results indicate that symptom modulation by sat-RNA C is mediated by the viral coat protein, and a putative interaction between the 3... |
