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Expression of sodium pump subunit genes during mouse preimplantation development

Posted on:1997-02-25Degree:Ph.DType:Dissertation
University:The University of Western Ontario (Canada)Candidate:Macphee, Daniel JamesFull Text:PDF
GTID:1464390014983840Subject:Biology
Abstract/Summary:
he Na,K-ATPase or sodium pump is an enzyme that is found in most animal cells. The enzyme pumps sodium and potassium ions against their concentration gradients and is hypothesized to be a mediator of fluid accumulation during mouse preimplantation development. The purpose of the present study was to determine, using reverse transcriptase-polymerase chain reaction (RT-PCR), whether all ;;;Using antisense oligodeoxynucleotides (ODNs), ;Sodium pump gene expression was also examined in another mammalian species, the horse, to provide further evidence that the enzyme is a mediator of mammalian blastocyst development.
Keywords/Search Tags:Sodium pump, Enzyme
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