| A total of 6 specific oligonucleotide probes were designed for the Ruminococci, 3 targeted to sites on 16S rRNA of Ruminococcus albus and 3 targeted to sites on 16S rRNA of Ruminococcus flavefaciens. A subspecies probe for Fibrobacter succinogenes S85 (Stahl et al., 1988) and a eubacterial kingdom probe (Stahl and Amann, 1991) also were used. Bacteria were grown as monocultures, dicultures and in triculture on cellobiose, cellulose and alkaline hydrogen peroxide-treated wheat straw to study competition among the three bacterial species on these substrates. The cellubiose-grown cultures were incubated at 39... |
