Rapid multiplication of cassava (Manihot esculenta Crantz.) as a potential method to provide disease-free plant materials

A major problem facing cassava production is availability of virus free stem cuttings, which are used for propagation. Research was conducted with various culture media to achieve rapid propagation of disease-free cassava (cv. Chilena) from shoot tip and nodal cuttings.;Shoot tip cuttings produced roots and grew vigorously as single shoots when cultured on half strength Murashige and Skoog (MS) medium supplemented with 2.5 ;Half strength MS supplemented with 1.0 ;Double phase of AMM and APM medium in reciprocal combinations showed that shoot tip explants formed roots and grew as single shoots when AMM was the solid phase. In contrast, nodal cuttings or shoot tips placed in APM solid phase developed callus with few roots.;When explants were cultured for 3 weeks in a double phase medium, with 0.05 ;A shoot tip explant, placed on AMM medium with GA...