| he insect cell baculovirus expression system has been widely accepted as an alternative to other expression systems (i.e. E. coli, Chinese Hampster Ovary, yeast) for the production of recombinant proteins. A procedure has been developed that significantly improves recombinant protein yields in Autographa californica nuclear polyhedrosis virus (AcMNPV) infected Spodoperta frugiperda cells. Specifically, this involves the exposure of these cells to high concentrations of thymidine prior to infection. Cells were incubated in medium supplemented with 50 mM thymidine for 12 hours prior to infection. The thymidine supplemented medium was removed immediately prior to infection. This strategy has been successful with secreated human placenta phosphatase and ;The effect of vector construction on protein expression was examined. |
