Synthesis Of Novel Bacterial Cellulosic Pocket With Medical Application Properties

This research focused on the synthesis of bacterial cellulosic pocket with dual functions of bacterial inactivation and drug storage by introducing rice bran and inulin as nitrogen sources and extra carbon source respectively.Rice bran as a nitrogen source was first investigated to confirm the bacterial live supportive characteristics in a Hestrin and Schramm(HS)Acetobacter xylinum culture media.The results indicated an unchanged composition of crude protein per increase in incubation days while diluted protein increased with the incubation period.Fourier transform infrared spectroscopy(FTIR)results showed the absorbance of OH groups.It was confirmed that the BC yield was directly proportional to the rice bran content.A general observation indicated a gradual transformation from a highly crystalline structure to a very amorphous structure as rice bran content increased with the endothermic decomposition of samples being recorded between 113.5? and 125.6?.Owing to the increase amorphous regions due to the presence of rice bran,Inulin as an external carbon source was then introduced to the fructose permeates of Gluconacetobacter xylinus(ATCC 10245)bacterial strain to successfully synthesize empty cellulosic pockets that could serve as drug storage.FTIR analysis confirmed a slight variation in the hydrogen bonds at 2893 cm-1 due to CH and CH2 stretching as a result of the rearrangement of the cellulose chains and hydrogen-bonding pattern.Conformational analysis revealed that inulobiose extending all-trans conformation corresponded to ?=?=?=180° whereas the fructofuranose moiety,the anomeric carbon was defined as C2.The definition of torsional angles ?=(?)(C1-C2-0-CI'),?=(?)(C2-0-C 1'-C2'),and ?=(?)(0-C1'-C2'-0')followed the proposed convention.Glycosidic angle C1'0 1'C2' was taken to be 114.0° and both O6 hydroxyl groups in inulobiose were fixed in a transposition,compelling the hydroxyl group to sterically favor comparison with the gauche orientation as found in the sucrose structure.Crystallography analysis results indicated a typical cellulose type I,while DSC and TGA analysis revealed a highly stable pocket until about 260?.Investigation into cellulosic pocket bacterial susceptibility against S.aureus and E.coli revealed a successful inactivation of S.Aureus and E.coli in the presence of photons while cellulosic pocket drug uptake test revealed a highly absorbent structure with the thermal stability directly proportional to the increase in drug uptake.This research is of great potential due to the multiplicity of doors it opens to the field of BC research and functions.Among these opportunities are in the provision of an easy alternative to the field of bacterial inactivation using common sugar.It also helps in driving down the cost of bacteria cellulose(BC)production and bacterial inactivation chemistry.