| Exposure to ionizing radiation may occur in accidental events and radiotherapy,and such exposures could be responsible for temporary and in some cases,permanent damages to the organs.Therefore,it is of special significance for researchers to develop highly sensitive,and efficient methods for the detection of biomarkers which could be utilized for the assessment of radiation induced damage.Surface-enhanced Raman spectroscopy(SERS)in the past few years,has attracted the researchers due to its broad sensing technology of target analytes even at single molecule level.With parallel advancement in preparation of highly efficient SERS-related nano-materials,researchers have made a lot of progress in developing SERS biosensors for the detection of disease specific biomarker,pesticides,antibiotic,and drug residues.This thesis is subjected to report the preparation and application of two kinds of SERS substrates:colloidal SiO2@Au(core/shell)NPs,and(2)Anodic alumina templates(AAO)based Au-NPs array.The main utilization of these materials and application findings of the experiments are as follows(1)AAO templates based Au-NPs array were utilized in direct and label-free analysis of serum of mice exposed to ionizing radiation.The Raman signals of myoglobin biomarker after different doses were analyzed,and radiation damage as a function of post-irradiation time was established(2)Aptamer-functionalized Au-NPs array were utilized in label-free,quantitative,and specific detection of inflammatory cytokine(Interleukin-6)as a result of radiation inflammation,drug and bacterial infection with a limit of detection at 0.8 pM and 10-12-10-7 M linear detection range(3)Aptamer-functionalized colloidal SiO2@Au-NPs were utilized via sandwich approach for the highly-sensitive detection of exosomal miRNA as a result of exposure to ionizing radiation.The quantitative measurement of sandwich approach was demonstrated in 10 fM to 1 nM linear range and 8.2 fM LOD. |
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