| Hybrid rice is an essential food crop,plays an important role in crop production.It is important to improve the utilization value of hybrid rice old seeds and the tolerance of low temperature,waterlogging and their compound stress during rice direct seeding.Improving the seed vigor can increase seedling percentage and the resistance of stress in field,finally increase the yield of hybrid rice.Seed vigor can be improved by seed treatments,seed priming is one of the rapidly developing met hods of seed treatments.Hybrid rice cv.Y liangyou 689(YLY689)old seeds and cv.Lingyouhuazhan(LYHZ)seeds were used as materials to investigate the effects of Fe-Zn-NA chelates priming on seed vigor,physiological and biochemical changes,proteomics under low temperature,waterlogging and their compound stress.Themain results were as follows:The effects and mechanism of Fe-Zn-NA chelates priming on old seed germination of hybrid rice were investigated.Fe-Zn-NA chelates priming significantly increased the seed vigor and viability of YLY689 old seeds.As compared to non-primed control,th e germination percentage was increased from 77.9%to 87.4%,germination index was increased from 11.48 to 16.99.Fe-Zn-NA chelates priming promoted the gene expressi onof?-amylase,peroxidase(POD),catalase(CAT),ascorbate peroxidase(APX)and ho rmone metabolism,significantly increased the activity of?-amylase,POD,CAT,APX,the content of sucrose,glucose,gibberellin(GA)and the rate of GA/ABA,significantl y reduced the contents of abscisic acid(ABA)and malondialdehyde(MDA).Proteome analysis showed that,the differential proteins of Fe-Zn-NA chelates priming on regula ting seed germination were about ribosome,RNA transport,protein processing,ubiquiti n mediated proteolysis,phagosome,peroxisome and metabolism of amino acid,nucleot ide,lipid,sucrose and starch,mitochondrial electron transport,glycolysis,TCA cycle.Fe-Zn-NA chelates priming promoted the up-regulation of ribosomal protein,aquaporin,serine-type carboxypeptidase,?-amylase,?-amylase,sucrose synthase,?-1,3-glucanase,phenylalnine ammonialyase,arginine decarboxylase,lipoxygenase,isocitratelyase,POD,CAT and the expression of their coding genes,These results indicated that Fe-Zn-NA chelates priming could increase the seed vigor and viability of hybrid rice old seeds.The effect and mechanism of Fe-Zn-NA chelates priming on seed germination of hybrid riceseeds under low temperature(5°C)were investigated.Fe-Zn-NA chelates priming significantly increased the seed vigor and viability of LYHZ seeds of recovery growth after low temperature.As compared to non-primed control,the germination percentage was increased from 82.7%to 96.0%,germination index was increased from 18.41 to 31.73.Fe-Zn-NA chelates priming promoted the gene expression of?-amylase,POD,hormone metabolism and low temperature response genes,significantly increased the activity of?-amylase and POD,the contents of soluble sugar,GA,auxin(IAA)and the rate of GA/ABA,significantly reduced the content of ABA and hydrogen peroxide(H2O2).Proteome analysis showed that,the differential proteins of Fe-Zn-NA chelates priming on regulating the resistance to low temperature during seed germination were about ribosome,protein processing and the metabolism of amino acid,sucrose and starch,mitochondrial electron transport,lignin synthesis,fermentation,TCA cycle,redox of ascorbic acid and glutathione.Fe-Zn-NA chelates priming promoted the up-regulation of ribosomal protein,RNA,NAD,ATP binding,G-box factor,alcohol dehydrogenase,serine-type carboxypeptidase,?-amylase,fructokinase,malate dehydrogenase,hexokinase,chitinase,pyruvate deaminase,POD,APX and the expression of their coding genes.These results indicated that Fe-Zn-NA chelates priming could improve the resistance of hybrid rice seeds to low temperature stress and the recovery growth ability of hybrid rice seeds after low temperature stress.The effect and mechanism of Fe-Zn-NA chelates priming on seed germination of hybrid rice under waterlogging were investigated.Fe-Zn-NA chelates priming significantly increased the seed vigor and viability of LYHZ seeds of recovery growth after waterlogging.As compared to non-primed control,the germination percentage was increased from 67.0%to 93.3%,germination index was increased from 17.63 to 27.88.Fe-Zn-NA chelates priming promoted the gene expression of?-amylase,POD,CAT,hormone metabolism and waterlogging response genes,significantly improved the activity of?-amylase,POD and CAT,increased the contents of soluble sugar,GA and the rate of GA/ABA,significantly reduced the contents of ABA,superoxide anion(O2-)and MDA.Proteome analysis showed that,the differential proteins of Fe-Zn-NA chelates priming on regulating the resistance to waterlogging seed germination were about ribosome and the metabolite of amino acid,nucleotide,lipid,sucrose and starch metabolism,mitochondrial electron transport,glycolysis,TCA cycle.Fe-Zn-NA chelates priming promoted the up-regulation of ribosomal protein,RNA,ATP binding,NADH dehydrogenase,chitinase,serine-type carboxypeptidase,lipoxidase,?-glucosidase,pyruvate kinase,aspartic-type endopeptidase,?-amylase,?-amylase,POD and the expression of their coding genes.These results indicated that Fe-Zn-NA chelates priming could increase the resistance of hybrid rice seeds to waterlogging stress and the recovery growth ability of hybrid rice seeds after waterlogging stress.The effect and mechanism of Fe-Zn-NA chelates priming on seed germination of hybrid rice under low temperature,waterlogging compound stress were studied.Fe-Zn-NA chelates priming significantly increased the seed vigor and viability of LYHZ seeds of recovery growth after compound stress.As compared to non-primed control,the germination percentage was increased from 81.0%to 93.2%,germination index was increased from 19.79 to 28.43.Fe-Zn-NA chelates priming promoted the gene expression of?-amylase,POD,CAT,APX,superoxide dismutase(SOD),hormone metabolism and response genes of low temperature or waterlogging,significantly increased the activity of?-amylase,POD,CAT,APX,SOD,and the content of soluble sugar,GA and the rate of GA/ABA,significantly reduced the content of MDA.Proteome analysis showed that,the differential proteins of Fe-Zn-NA chelates priming on regulating the resistance to compound stress during seed germination were about ribosome,protein processing and the metabolite of amino acid,phenolics,lipid,sucrose and starch metabolism,mitochondrial electron transport,lignin synthesis,redox of ascorbic acid and glutathione.The result of the gene expression about differential proteins show that,Fe-Zn-NA chelates priming promoted the expression of ribosomal protein,metal cation binding,calcium binding cytochrome C,S-adenosylmet hionine synthetase,ubiquitin enzyme,phenylalanine decarboxylase,?-glucosidase,fructokinase,chitinase,serine-type carboxypeptidase,cytochrome oxidase and cysteine endonuclease,?-amylase,?-amylase,POD,APX and the expression of their coding genes.These results suggested that Fe-Zn-NA chelates priming could increase the resistance of hybrid rice seeds to compound stress and the recovery growth ability of hybrid rice seeds after compound stress.In conclusion,after Fe-Zn-NA chelates priming,with increasing the activity of antioxidant enzyme and weakening lipid peroxidation,the level of starch and GA metabolism were en hanced.Finally,the seed vigor and viability of hybrid rice old seeds and the hybrid rice seeds under low temperature,waterlogging and their compound stress were increased,and increased the resistance of hybrid rice seed to low temperature,flooding and complex stress... |
PDF Full Text Request