| ObjectiveThere is symbiosis and balance between microecology and host.Regulating the microecology of flora is an effective target for the treatment of diseases.The application of traditional Chinese medicine preparation and fecal bacteria transplantation(FMT)can effectively regulate the microecology of the host flora and restore the physiological functions of the host.In this study,we observed the effect and pathway of the dried ginger jujube prescription on spleen deficiency syndrome and spleen-stomach cold syndrome in rats.Observe the effect of oral yeast and probiotics on microecology of digestive tract microflora in rats.Spleen deficiency and cold syndrome of spleen and stomach deficiency belong to the category of sub health state(SHS).This study combined the theory of qi,blood,body fluid,and applied the digital and quantitative identification of sub health state of human body with the TCM Auxiliary Diagnosis and Treatment Performer(TCM-ADTP).Combined with Low Frequency and Negative Pressure Viscera-Meridian Rectifier(LFNPVMR),a new regulate method of digitizing and quantifying the sub health state of human body was put forward.Method1.SD rats were randomly divided into blank group,spleen deficiency group,spleen and stomach cold group,spleen deficiency drug group,spleen and stomach cold drug group,blank drug group,6 in each group.Experimental rats in each drug group use dried ginger jujube prescription gavage three days at 9 am in advance.Drug gavage continued during the experiment.The blank group was not treated.After each drug group use drug gavage three days,spleen deficiency group and spleen deficiency drug group,use tap water(0℃)gavage at 3pm every day.Spleen and stomach cold group and spleen and stomach cold drug group use vinegar(0℃)gavage at 3pm every day.Dried ginger jujube prescription treatment for 13 days.The bacterial diversity and composition of cecum contents in each group were analyzed by 16SrRNA high-throughput sequencing.The serum levels of vasoactive intestinal peptide(VIP),somatostatin(SST),5-hydroxytryptamine(5-HT),corticotropin releasing hormone(CRH)and gastrin(GT)were determined by ELISA.The content of D xylose in serum was determined by colorimetry.The composition and content of dried ginger in serum of experimental rats were determined by liquid-mass chromatography.2.SD rats were randomly divided into a blank group,a yeast group and a probiotics group,6 in each group.In the yeast group and the probiotics group,rats were given yeast/probiotics gavage once every day at 9:00 a.m.for a total of 6 days.The blank group was not treated.16SrRNA high-throughput sequencing technique was used to analyze the diversity and composition of bacteria in digestive tract of rats in each group.3.Collected from April 2016 to December 2017,there were 122 cases of students from BUCM(Beijing University of Traditional Chinese Medicine,BUCM)and employees of a certain company who met the requirements of SHS and Health state(HS).After TCM-ADTP diagnosis,30 patients in qi deficiency group,32 patients in qi stagnation group,30 patients in blood deficiency group and 30 patients in healthy group.In the qi-deficiency group,there were 11 males and 19 females.The youngest is 20 and the oldest is 34.In the qi stagnation group,there were 10 males and 22 females.The youngest is 21 and the oldest is 43.In the blood deficiency group,there were 12 males and 18 females.The youngest is 19 and the oldest is 46.In the healthy group,there were 12 males and 18 females.The youngest is 21 and the oldest is 43.qi deficiency group,qi stagnation group,blood deficiency group were compared with healthy group,and there was no statistical difference in gender and age(P>0.05).TCM-ADTP was used to collect and analyze the "look,smell,ask and cut" information of qi deficiency group,qi stagnation group,blood deficiency group and health group,give recommend appropriate prescriptions.4.Collected from September 2016 to September 2017,there were 51 cases of students from BUCM and employees of a certain company who met the requirements of SHS.After TCM-ADTP identification and diagnosis,there were 25 patients in meridian conditioning group and 26 patients in viscera conditioning group.In the meridian conditioning group,there were 10 males and 15 females,the youngest is 23 and the oldest is 34.In the viscera conditioning group,there were 10 males and 16 females,the youngest is 19 and the oldest is 46.There was no statistical difference in gender and age between the two groups(P>0.05).TCM-ADTP was used to collect and analyze information of "look,smell,ask and cut" before and after the conditioning of meridian conditioning group and viscera conditioning group.LFNPVMR was applied to the meridian conditioning group and viscera conditioning group.5.Collected from September 2016 to September 2017,there were 35 cases of students from BUCM and employees of a certain company who met the requirements of SHS.TCM-ADTP identified 20 patients with qi deficiency syndrome and 15 with blood deficiency syndrome.Among them,there were 15 males and 20 females,the youngest is 19 and the oldest is 46.TCM-ADTP was used to collect and analyze information of "look,smell,ask and cut" before and after the conditioning of qi deficiency and blood deficiency.LFNPVMR was used to regulate qi deficiency and blood deficiency.Results1.Under the continuous stimulation of tap water(0℃),spleen and stomach qi deficiency,transportation and transportation disorders,formed the spleen deficiency syndrome model.Under the continuous stimulation of vinegar(0℃),the experimental rats were filled with cold and dampness,and the spleen Yang was blocked,forming the model of spleen and stomach cold syndrome.Experimental rat under the tap water(0℃)stimulation of intestinal flora in the relative abundance of the genus level compared with the blank group,the relative abundance of Lactobacillus decreased,Dorea,rc4-4,Christensenella,Alistipes,Bilophila,Coprobacillus and Paraprevotella relative abundance increased(P<0.05).Experimental rat under the vinegar(0℃)stimulation of intestinal flora in the relative abundance of the genus level compared with the blank group,Lactobacillus and Streptococcus decreased,Bilophila,Candidatus_Arthromitus,Facklamia and Marvinbryantia increased(P<0.05).Taking dried ginger jujube prescription can improve the symptoms of mental fatigue,weak and diarrhea with spleen deficiency(P<0.05).Taking dried ginger jujube prescription can improve the symptoms of abdominal distension,diarrhea and chills in experimental rats with spleen and stomach cold(P<0.05).Taking dried ginger jujube prescription impact experimental rat under the tap water(0℃)stimulation of intestinal flora in the relative abundance of the genus level,Lactobacillus increased,Ruminococcus,Bifidobacterium,Dorea,Phascolarctobacterium,rc4-4,Christensenella,Alistipes,Bilophila and Paraprevotella decreased(P<0.05).Taking dried ginger jujube prescription impact experimental rat under the vinegar(0℃)stimulation of intestinal flora in the relative abundance of the genus level,Clostridium,Anaeroplasma and Marvinbryantia decreased,Odoribacter,Anaerofustis and Holdemania increased(P<0.05).Taking dried ginger jujube prescription increased experimental rat cecum contents pH value under the vinegar(0℃)(P<0.05).Taking dried ginger jujube prescription failed to effectively improve experimental rats serum VIP,SST,5-HT,CRH,GT and D xylose level under the stimulus of tap water(0℃)and vinegar(0℃)(P>0.05).After taking dried ginger jujube prescription,the serum of experimental rats contained low concentration of 6-gingerol.2.After experimental rats oral yeast,the relative abundance of the genus level in the oral cavity Aggregatibacter,Pseudomonas,Asticcacaulis,Comamonas and Aminobacter decreased,while the relative abundance Streptococcus,Veillonella,Gemella and Turicibacter increased.The relative abundance of the genus level in the Gastric contents Acinetobacter,Rheinheimera,Comamonas,Agrobacterium,Adlercreutzia and Devosia decreased,while that of Gemella increased.The relative abundance of of the genus level in the Intestinal contents Veillonella and Gemella increased.The relative abundance of the genus level in the Cecum contents Lactobacillus,Turicibacter and SMB53 increased,while that of Phascolarctobacterium and Desulfovibrio decreased(P<0.05).After experimental rats oral probiotics,the relative abundance of the genus level in the oral cavity Streptococcus.Veillonella,Lactobacillus,Enhydrobacter,Gemella,Turicibacter and Marinobacter increased,while the relative abundance Granulicatella decreased.The relative abundance of the genus level in the Gastric contents Lactobacillus,Clostridium,Turicibacter and Anaeroplasma increased,while that of Acinetobacter,Rheinheimera,Agrobacterium,Morganella,Devosia and Cloacibacterium decreased.The relative abundance of the genus level in the Cecum contents Phascolarctobacterium,Desulfovibrio and Facklamia decreased,while that of SMB53 and Lachnospira increased(P<0.05).3.Compared with the healthy group,people in the qi deficient group had lower pulse strength,increased the proportion of tongue ecchymosis(P<0.05).The proportion of five-tone physique identification metal type is the largest.The prescription is recommended for decoction of four mild drugs,pill of costus and amomum with six noble ingredients,Yangxin soup,increasing exercise time,maintaining adequate rest time,taboo coffee,strong tea and other stimulant drinks.Compared with the healthy group,people in the qi stagnation group had higher pulse string tightness,faster pulse wave velocity,increased proportion of deep red tongue and increased proportion of tongue ecchymosis(P<0.05).The proportion of five-tone physique identification earth type is the largest.The prescription is recommended for longdan xiegan decoction,chuan xiong cha diao san,chaihu shugan powder,tianma gouteng drink,Loosen body and mind.Compared with the healthy group,people in the blood deficiency group had lower pulse strength,faster pulse wave velocity,increased proportion of red tongue,increased proportion of the yellow moss,increased proportion of tongue ecchymosis(P<0.05).The proportion of five-tone physique identification metal type is the largest.The prescription is recommended for guipi decoction,ma xing shi gan decoction,eat more fruits and vegetables,increase your exercise time.The healthy group had the highest proportion of flat pulse and light red tongue.The proportion of five-tone physique identification metal type is the largest.4.SHS after meridian conditioning,pulse pressure decreased,pulse strength increased,pulse string tightness decreased,proportion of light red tongue increased,proportion of deep red tongue decreased,and proportion of tongue ecchymosis decreased(P<0.05).There was no statistically significant difference in five-tone body recognition before and after conditioning(P>0.05).Before conditioning the proportion of five-tone physique identifying soil type was the largest.After conditioning the proportion of five-tone physique identifying wood type was the largest.After meridian conditioning,the chief complaint symptoms(strong neck pain,fatigue,dream more)were all alleviated,and 22 patients with SHS(liver depression syndrome,internal heat syndrome,qi deficiency syndrome,and heart and spleen deficiency syndrome)were converted to HS(P<0.05).SHS after viscera conditioning,pulse pressure decreased,pulse fluency increased,the proportion of light red tongue and red tongue increased,the proportion of deep red tongue decreased,and the proportion of tongue ecchymosis decreased(P<0.05).There was no statistically significant difference in five-tone body recognition before and after conditioning(P>0.05).Before conditioning the proportion of five-tone physique identifying metal type was the largest.After conditioning the proportion of five-tone physique identifying metal type was the largest.After viscera conditioning,the chief complaint symptoms(fatigue,dream more,restlessness,strong neck pain)were all alleviated,and 23 patients with SHS(liver depression syndrome,internal heat syndrome,qi deficiency syndrome,and heart and spleen deficiency syndrome)were converted to HS(P<0.05).There was no statistical difference between the curative effect of meridian conditioning and viscera conditioning(P>0.05).5.Qi deficiency,blood deficiency patients after meridian-viscera conditioning,pulse pressure decreased,pulse strength increased,and the proportion of tongue ecchymosis decreased(P<0.05).There was no statistically significant difference in five-tone body recognition before and after conditioning(P>0.05).Before conditioning the proportion of five-tone physique identifying metal type was the largest.After conditioning the proportion of five-tone physique identifying metal type was the largest.After meridian-viscera conditioning,the chief complaint symptoms(strong neck pain,fatigue,dream more,restlessness)were all alleviated,and 32 patients with SHS(qi deficiency syndrome,blood deficiency syndrome)were converted to HS(P<0.05).Conclusion1.Oral administration of dried ginger jujube prescription improved spleen deficiency syndrome and spleen-stomach cold syndrome by regulating the intestinal flora of experimental rats,and found a new pharmacodynamic pathway for dried ginger jujube prescription.2.Oral yeast/probiotics can improve gastrointestinal motility and reduce inflammation by regulating microecology of digestive tract flora in experimental rats.3.TCM-ADTP can be applied to the digital identification of the human body’s qi and blood state,and the corresponding prescription suggestions are proposed.4.The application of LFNPVMR in SHS meridian conditioning and viscera conditioning,the effect is significant,worthy of clinical application.5.The application of TCM-ADTP can digitize and quantify the identification of qi deficiency and blood deficiency syndrome,and LFNPVMR can regulate the qi deficiency and blood deficiency syndrome by the meridian-viscera conditioning,with remarkable effects.6.There is a certain correlation between the microecological structure of the microflora and the theory of qi,blood and body fluid in Traditional Chinese Medicine. |
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