Analysis Of Promotor Character And Expression Pattern Of Of FIS Class Polycomb Genes

Genomic imprinting is a genetic phenomenon by which certain genes are expressed in a parent-of-origin-specific manner. It is an inheritance process independent of the classical Mendelian inheritance. Imprinted genes are either expressed only from the allele inherited from the mother, or in other instances from the allele inherited from the father. Forms of genomic imprinting have been demonstrated in insects, mammals and flowering plants(eg. FIE,FIS1/MEA and EMF2 in Arabidopsis thaliana; FIE1,FIE2,Mezl and Mez2 in Zea mays).As one of the most important crops of the world, Oryza sativa is a model of monocotyledon plant. The endoperm is the main edible part of rice which takes a crucial role in the embryo development. It is useful to carry out some necessary researches on PcG genes of rice, because it is helpful to understand the mechanism of endosperm development in cereal which has a potential value in breeding.For the purpose of making clear the mechanism of PcG genes of rice. I try to make it clear in two different ways.In one hand,bioimformation method was used to analyst the promoter of PcG genes in rice. After that I construct the expression vector use two vector which contain GFP and GUS.In the other hand, positive and negative crossbreed was used to study the expressing of FIS class gene.After crossbreeding in 9311 and Nipponbare, I take inflorescences of rice after pollination, and separate the endsperm and embryo. Then by the tool of RT PCR, I can inspect the expression level of FIS class gene. And the result indicates that gene FIE2b and EZ1 maybe is related to genome imprinting.