The Stress Treatment Research Of DREB,bZIP Class Of Transcription Factor In Peanut(arachis Hypogaea L.)

In its growth process of plants are often subjected to different degrees of biotic and abiotic stress, these stress seriously affect plant growth and development, and agricultural production tremendously negative impact. Study found that long-term process of biological evolution; plants have developed their own resistance to drought stress a variety of mechanisms to regulate itself through physiological and metabolic changes in resistance to drought. One important mechanism is through the environmental stress signal transduction to activate gene expression in response to environmental stress. In stress signal transduction, transcription factors regulate several stress response due to the expression of genes of concern.We export large Luhua peanut seed mix of 14 different developmental stages in the cloned cDNA library identified the three peanut AP2/EREBP genes, named PNDREB1, PNTINY1,PNTINY2 and a bZIP genes, named PNbZIP1.As a class AP2/EREBP family members,DREBs specific transcription factor present in plants,involved in plant drought, high salinity and low temperature and other abiotic stress response and response.In plants,bZIP transcription factor involved in seed storage protein gene expression,light organ morphogenesis and control related built.Research PNDREB1,PNTINY1, PNTINY2,PNbZIPl'and applied to practice on improving the quality of plants, improving plant resistance has very important significance.This paper has made the major findings are as follows:1, by RT-PCR method on PNDREBl,PNbZIP1 were non-expression pattern analysis of biological stress, and PNbZIP1 expression and induction of organized expression pattern analysis. Expression pattern of the PNDREB1 analysis revealed that the gene can produce responses to low temperature treatment, low temperature expression doubled; expression levels induced by salicylic acid is enhanced, but not obvious to ethylene response, and gene expression is regulated by jasmonic acid a fat suppression. Expression pattern of the PNbZIPl study shows that in peanut roots, stems, leaves,flowers,seeds,fruit needle was expressed in both the constitutive expression, and in flowers and young seeds in the higher expression; by low temperature, ethylene, salicylic acid, a more rapid response, but Nacl, jasmonic acid methyl ester, ABA response is not obvious. PNTINY1 able to respond Nacl, low temperature, ethylene jasmonic acid methyl ester, ABA treatment conditions, did not respond to salicylic acid. Very strong level of ethylene response. Jasmonic acid methyl ester under the conditions of decreased responsiveness.PNTINY2 able to respond Nacl, low temperature,ethylenejasmonic acid methyl ester,ABA,salicylic acid treatment conditions. Jasmonic acid methyl ester under the conditions of the processing time to respond to the increase with the degree of reduction, while measuring less than 48 hours to check the expression. Acid conditions, PNTINY2 responsiveness gradually increased.2,using yeast one-hybrid system and the activation energy PNbZIP1 binding were identified, the results show:PNbZIP1 cannot ABRE element binding, but the transcriptional activation activity. Containing the DRE cis-element using the yeast one-hybrid system verification PNTINYl,PNTINY2 binding and activation activity, the results show that both can and DRE cis-element binding and transcriptional activation activity.3,Ti transgenic tobacco on behalf of PNDREB1 After further screening by the subculture to obtain T2 generation of PNDREB1 antisense transgenic tobacco plants were six lines. Construction PNbZIP1 of sense and antisense plant expression vector pCAMBIA1301-PNbZIP1(+)-GUS and pCAMBIA1301-PNbZIP1(-)-GUS, used for tobacco transformation. There has been a generation of PNbZIP1 T2 antisense transgenic lines each 10. T1 generation of PNTINY1,PNTINY2 transgenic tobacco by further screening after subculture, were T2 generation of PNTINYl sense and antisense transgenic tobacco lines of all six, were PNTINY2 T2 generation of transgenic tobacco antisense lines 2.4,the obtained T2 generation of PNDREB1,PNbZIPl transgenic tobacco to non-biological stress on plant stress tolerance analysis, we found PNDREB1 (-) to low temperature stress than the sensitive, low temperature, the transgenic plants, stress-related gene expression and physiological changes in both different from the wild type, suggesting that the gene in low-temperature signal transduction in response and the role of the gene while ABA,Nacl,drought stress has a certain response, that the gene in plant resistance to abiotic stress has a comprehensive certain functions.PNbZIPl stress tolerance test in progress.This study reveals PNDREB1,PNTINY1,PNTINY2,PNbZIP1 in non-biological role and function under stress, and they stress signal transduction pathway in the regulation mechanism provides a good clue for the further study the functions of these two genes lay a good working basis. Means of fostering the adoption of integrated molecular breeding to improve resistance to new varieties of genetically modified resilience provides a theoretical basis and excellent genetic resources.