Characterization And Expression Analysis Of VERNALIZATION1 Gene In Brachypodium Distachyon L. And Chip Data Against The Photoperiodic Flowering In Wheat

Vernalization, the requirement of a long exposure to low temperatures to accelerate flowering, after vernalization plants will not immediately flowering,but shoot apical meristem of plants will be had flowering potential,which is an important physiological phenomenon. Wheat as important crops, its molecular mechanism of vernalization relative to Arabidopsis is not yet clear, but studying them is difficult by themself. Brachypodium distachyon L. with characteristics of vernalization and easily transformed will be a new model system for the temperate grasses,and be used in Research about the function of VRN1 gene.The VRN1 homologs was isolated from Brachypodium, and designated as BdVRN1, which coding sequence of the MADS-box and K-box conserved domains, is a MADS-box gene family member. Non-coding sequence of BdVRN1 gene about promoter and introns which may be important to vernalization and photoperiod. The results of real-time quantitative RT-PCR experiment indicated that through the vernalization treatments, we can find that BdVRN1 gene expression can be improved,indicates that the BdVRN1 gene expression can be regulated in different plant organs by the vernalization; During the vernalization treatment,we find that BdVRN1 gene can be increated under the long-day and short-day conditions together,Indicates that BdVRN1 gene can be induced to expression under LD and SD conditions with vernalization. At the end of the vernalization treatment, BdVRN1 gene is continue increated under the long-day condition, but BdVRN1 gene is depressed under the short-day condition, Uner the two conditions, the different expression of BdVRN1 gene influence flowering time and plant morphology, suggest that BdVRN1 gene by vernalization may play an important role in flowering; Meanwhile, there are some different expressiong of BdVRN1 gene in different continuous conditions about Day Length---BdVRN1 gene function by the regulation of photoperiod. The different expression of BdVRN1 gene influence flowering time and plant morphology, suggest that BdVRN1 gene by photoperiod may also play an important role in flowering; In addition, through the anlysis of the BdVRN1 expression from dark to light, indicates that it can be regulated under different daylength conditions in circandian clock. These results are illustrated that BdVRN1 gene by vernalization and photoperiod effects of dual regulation, BdVRN1 gene expression is closely linked and showed some correlation with the flowering,so BdVRN1 gene may be an important gene affect the Flowering.The transfer of wheat on the short day to long day condition when there is a induced signal of flowering and a signal pathway to start the flowering process, using Agilent gene chip (Shanghai Bio) analysis of this process may be found differentially expressed genes, which were the early response genes of photoperiod pathway.According to chip in gene expression changes in selected candidate genes of Photoperiod in wheat,Through the chip data, a total of 43603 group probes have signals, with cluster3.0 software group screened a total of 2173 valid Fold change≥2, accounting for 5% of the total number of probe chips around.Then according to functional classification, and Photoperiod sequences were divided into 3 groups,①continue to rise (234) by Photoperiod;②up then down (104) by Photoperiod;③down (102) by Photoperiod, and each functional classification of the data, suggesting that these genes may be involved in the response of photoperiod signal process in wheat.