The Bioinformatics Analysis Of Several Functional Proteins' Autoinhibitory Domain And Their Mechanism

Autoinhibitory domain is a kind of distinct structure which exists ubiquitously in diverse proteins. In absence of stimulator, autoinhibition of activated proteins is a common mechanism for modulating cellar function of these proteins. We analyzed their autoinhibitory domain with bioinformatics techniques, such as alignment of entire sequence, alignment of sequence of autoinhibitory domain, phylogenetic analyses and tree construction, and membrane topologic analysis.P2B type atpases is P type ion-pump super family members, we collected information about autoinhibitory domain of 25 proteins, characterized this domain and made analysis of likely mechanism. Subsequently, we selected 8 proteins of P2BATPase of Arabidopsis. The results showed that highly conserved amino acids exist in autoinhibitory domain of P2BATPase. These conserved amino acids play important roles in autoinhibitory function. Moreover, the phylogenetic relationship of autoinhibitory domain can remarkable reflects the same relationship of the whole protein in some extent.Ca2+-dependent Protein Kinase (CRKS), to study the Autoinhibitory domain mechanism of it, we reference the Autoinhibitory domain sequences of CDPK which was studued clearly, Speculate the sequence of the Autoinhibitory domain of the CRKS,then make the tree construction of both the all sequence and the Autoinhibitory domain sequence, trying to restrain the Autoinhibitory domain and its mechanism from their conservative and revoltion.CaV1 family is a family including K+ voltage-gated channel and Na+ voltage-gated channel transmembrane ion channels protein super family members, We selected 4 proteins of CaV1 family, and analyzed their autoinhibitory domain with bioinformatics techniques, such as alignment of entire sequence, alignment of sequence of autoinhibitory domain, phylogenetic analyses, tree construction and membrane topologic analysis. The results showed that highly conserved amino acids exist in autoinhibitory domain of CaV1 family. These residues play important roles in autoinhibitory function. Moreover, the phylogenetic relationship of autoinhibitory domain can remarkable reflects the same relationship of the whole protein in some extent.