The Genome Sequence Of An Antibacterial Peptide Pro Ducing Marine Bacterium, Bacillus Subtilis Gt 1

Bacillus subtilis gt 1 is a strain of Gram-positive, marine bacterium that can produce antimicrobial peptide (AMP). This strain is closely related to the well studied model organism Bacillus subtilis str.168. Many genomes of the Bacillus subtilis strains have been sequenced, but no record of whole genome sequencing from marine bacteria is reported to our knowledge. We determined the draft genome sequence of the B. subtilis gt 1. isolated from the Indian Ocean using short reads around 40 base-pairs (bp) in length. By de novo assembly and reference-guided assembly combined with PCR sequencing methods, the draft was constructed. The genome sequence of B. subtilis gt 1 comprises 4,247,908 bp with an average G+C content of 44.8%, containing 4331 predicted protein-coding genes that have an average length of 844 bp,30 rRNAs(10 rRNA operons), and 77 tRNA genes.81.7% of the predicted B. subtilis gt 1 coding sequences have B. subtilis str.168 orthologs, but 444 protein-coding genes were not found in genomes of Bacillus genus. Of these protein-coding genes, some candidate genes and gene clusters that involved in novel ribosomal or non-ribosomal antimicrobial peptide production were identified. B. subtilis gt 1 provides a model Gram-positive bacterium for marine bacteria, it is of great importance for basic and applied researches.