Effect Of SO₂ Derivatives And RE On Voltage-dependent K+ And Na+ Current In Acutely Isolated Rat Neurons And Smooth Muscle Cell

China has the largest reserves of Rees in the world . Because the Rees have superior physical and chemical properties, they are widely utilized in various fields. But the biological effects has highlighted. The first part of this article are using the whole cell patch clamp technique comparison of lanthanum, gadolinium, ytterbium three kinds of rare earth ions on the dorsal root ganglion neurons membrane potassium current, investigation the effect of those three rares on DRG cell. The purpose of this study was to investigate the transient outward potassium current change by La³⁺,Gd³⁺,Yb³⁺, The results showed that: 10,100,500uM of La³⁺,Gd³⁺,Yb³⁺ inhibition of transient outward potassium current; 100uM of La³⁺Gd3+,Yb³⁺ on activation kinetics of IA obtained three kinds of rare earth ions change the curve to more positive with Progressive trend. LaCl₃,GdCl₃,YbCl₃ on the delayed rectifier potassium current showed that 100uM of La3+,Gd³⁺,Yb³⁺inhibition of IK in a dose dependent manner and in a voltage-dependent manner, same doses of three kinds rare earth inhabit the Ik in a progressive manner. on the activation kinetics of IK found that La³⁺ and Gd³⁺ nearly doesn't change the activation of Ik, except Yb³⁺, which shift the activation curve to more positive, and three kinds rare earth element decreased the activation slope of IK, the delayed rectifier potassium channel conductance decreased. LaCl₃,GdCl₃,YbCl₃ also can inhibit the transient outward potassium current and delayed rectifier potassium current, causing the primary sensory neurons in the hyperpolarization-excitability, and ultimately the feeling abnormal, this abnormal sensory afferent from peripheral nerve into central nervous system. The present date suggests that rare earth ions have neurotoxicity, and neurotoxicity of Yb³⁺stronger than La³⁺ and Gd³⁺. In order to investigate the bio-effects of Yb³⁺ on hippocampal neurons voltage-gated sodium current and its dynamic effects. Our result suggest that the biggest current of voltage-gated sodium channel changed from 2154±163pA to 2807±60pA, increased sodium current by 30.3 percent, without affecting the current inactivation curve and activation curve. At the prospect of sodium current time to peak and time parameter of inactivate, activation sodium channel time shortened, but sodium current evaluation by 30.3%, the result indicated that Yb³⁺ significantly increased the sodium current conducted, this maybe one of mechanism that the Yb³⁺ effect on hippocampal neurons.Sulfur dioxide is the main pollutant in the atmosphere, the whole world each year emits a million tons of SO₂ into the atmosphere, SO₂ is harmful on animals and plants are an indisputable fact. In the second of this article also study the sulfur dioxide derivatives on rat aorta smooth muscle cell membrane potassium ion channel effects by the whole cell patch clamp technique. In order to investigation the relationship of SO₂ pollution and cardiovascular diseases, such as arrhythmias, ischemic heart disease, pulmonary heart disease Etc. The results show that SO2 derivatives can increased IKv significantly, while the TEA and 4-AP can be partially inhibited this effect; Experimental study showed that 10μmol/L SO₂ derivatives can significantly shift the activation curve of IKv to hyperpolarizing. SO₂ derivatives applied on rat vascular smooth muscle cells, causing outward potassium current increases, making the cell membrane hyperpolarization, closure of the voltage-gated calcium channels. Blood vessels relaxed and blood pressure decreased. This maybe one of the mechanisms of SO₂ and its derivatives reduce the blood pressure.