| Thermophilic Archaea and thermophilic bacteria interest more and more scientists. Because, they can be a model system for the research that the biochemistry and molecular biology characteristic of the thermophilic life can be studied. They also influenced the evolutionary process of biogeochemistry and ecological system, including the sustenance circulation,the clans,the population composition of life,the hereditary transformation,evolutions and so on. The genome of bacteriophage is tiny, but it codes the proteins needed for the duplication of itself. For example, the DNA packaging proteins, head and tail adaptor, the duplication related proteins, transcord regulating proteins and the cracking related proteins. Through the comparative genomics,a lot of gene diversity data could be got. At the same time, the obvious similarity exists among the genomes was also found.The characteristic that thermophilic bacteria can get multiplication and loses no activity at high temperature contains huge application value at gene engineering, ferment industry, the anaerobic treat of wastewaster and flotsam, and the exploitation of mineral resource. So it makes a very important actuality meaning that the high temperature adaption mechanism could be understood.This study inoculates mud sample which is collected from latitude 14.7510, 44.9774W of deep-sea hydrothermal areas on the 2216E substrate. The samples are cultured at 60℃, 65℃, 70℃and 75℃to screen for lytic bacteriophage. And three kinds of drastic thermophilic bacteriophage were found after verification, one of them may be a new kind of thermophilic bacteriophage that has never been reported.According to the whole genome sequence of GVE2, the respective primes were designed and the ORF7 ORF8 ORF9 ORF10 ORF11 ORF33 ORF34 ORF37 ORF38 ORF39 were cloned into the expression vector pGEX-4T-2. The respective recombinant proteins were expressed in E coli. After purification, the recombinant proteins were subjected to get the antibody preparation, respectively. It's useful for further research about the function of the proteins and the understanding of phage-host interaction mechanism.This research verified the function of the ORF8 (head and tail adaptor) of thermophilic bacteriophage GVE2. Base on the sequence comparison on NCBI web tools, its function was predicted to be a head and tail adaptor of GVE2. After the purification of the purpose protein, the recombinant proteins were subjected to antibody preparation, ELISA experiment valued the efficiency of the antibody. Western blot results showed that the protein was expressed during the later stages of GVE2 infection, suggested it was the late genes of GVE2. Then the position in the phage was tagged through the immune electron microscopy experiment.To study the mechanism of high temperature adaption of thermophilic bacteria, this research used Thermus thermophilus WL screened from hot spring as the material, and perform the study though proteomics。The growth curve was drawed according to the growing situation of the superoxide dismutase anergy mutant type constructed before. The result revealed that the optimum growth temperature of the mutant type was 65℃, however the optimum growth temperature of the wild type was five℃higher, 70℃. Apart from this, the maximum growth temperature of the mutant type was 75℃, but for the wild type was 80℃. 2D-DIGE experiment was carried out to find out the proteins related to the thermophilic bacteria adaption of high temperature, successfully got some diversity points. The high temperature adaption related proteins were preliminarily checked out though mass spectrum technology.
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