| In order to reveal the regularity of archaea communities changes in spring on multiple time scales in seismic fault belt spring water in Urumqi and determine the geochemical factors which impact of change of archaeal community, the 10th spring water in Urumq was sampled periodically, we monitoring the geochemistry elements, such as hydrogen, radon, methane, sulfide, methane, carbon dioxide, helium, fluorine and hydrarg, et al. Microbial communities were collected by microporous membrane filtration, then total DNA were directly extracted by SDS-enzymatic disruption method and the V3 region of the Archaeal 16S rDNA gene amplified by Semi-nest-PCR. the optical density of bands from the information and monitored geochemical indicators was analysis by the principal component analysis separate。And then they was analysed by canonical correspondence analysis together. We choose three time scales of this experiment: (1) the time scale of months : samples selected from September 2th,2009 to September 29th ,10 samples in all. (2) time scale of season : from July 4th, 2009 to July 31th, October 2th, 2009 to October 29th, January 3th, 2010 to January 30th and April 5th, 2010 to April 30th . four-month in all. (3) time scale of year : we picked out the samples from July 2, 2009 to June 26,2010 witch corresponds to 24 solar terms. At the same time , all the samples come from July , 2009 to June, 2010 , whose archaeal community structure were used to canonical correspondence analysis with hydrogeochemical factors . The results reveal that: during the whole period of experiment, the spring water, the archaeal community structure exhibited an important temporal variability whatever the time interval considered. PCA analysis revealed a hierarchy in the temporal variability of bacterial community: mouth |
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