Research Of Cell Capture, Manipulation And Optical Detection Platform

Cell research is an important part in disease research such as cancer and AIDS. It will help to develop cell biology to give a convenient and fast cell capturing and detecting method. It is meaningful to use the MEMS technology to research cell biology and biological device miniaturization.This paper uses MEMS technology to fabricate a kind of cell capture chip based on immunomagnetic bead technology, proposes a platform which uses MEMS cantilever to manipulate cell and uses capacitance micro-force sensor to test force, and develops a kind of optical detection system for detecting cell growing condition. The main contents include as following:1. Magnetic capture theory, Bio-MEMS chip's performance simulation and its fabrication process is introduced, and this chip's function test using cancer cells and CD4 cells is discussed. The cancer cells'capture efficient is 92.3% at the 0.1ml/h rate.2. The fabrication of MEMS capacitance which is used to test cell's micro control force is introduced. Their mechanical and electrical properties simulation and the canpacitance test circuit is discussed.3. The optical detection system for cells growing condition test by Micro Computer Unit, Arduino development board, VB, LED and PD is set up. The environment test, blue light test and yellow dye solution test is discussed.This MEMS cell capture chip needs only small volume of sample, and is operated easily. The cell manipulation platform composed of MEMS cantilever and capacitance micro-force sensor is useful for cell research. And the optical detection system for cell's growing condition test could promote the miniaturization of biological testing equipment.