| Neuropathic pain is the pain initiated or caused by a primary lesion or dysfunction in the nervous system. Nowadays, we still have no effective medicine can treat this disease specificially because the mechanisms underlying this clinical chronic pain are still not totally understood. In order to reveal the functions of nerve injury induced gene expression changes in primary sensory neurons (dorsal root ganglion, DRG) and DRG neuron cell death, we focused on three projects including high profile proteins screening project, Bax project and Db/Db project. We randomly selected 797 antibodies against 725 proteins from Human Atlas Program and performed immunohistochemistry and Western Blot to screen proteins that changed their expression in Sprague Dawley rat's DRG neurons after 1 week of axotomy. Though IHC result showed that 31 proteins have clear staining in DRG neurons including 9 up-regulated and 5 down-regulated proteins in ipsilateral DRG neurons, only ATF3, CECR5, LYN, UPF3B 4 up-regulated proteins were confirmed by western blot and SCGN was down-regualted. Bax is a key factor in mediating neuron cell apoptosis in various neurodegenerative diseases and during the development of nervous system. We counted DRG neuron numbers from Bax KO and Wild Type mice after 1 week and 1 month of axotomy by performing LSCM based stereological method to investigate whether Bax is involved in axotomy-induced DRG neuron cell loss. The result shows that Bax is involved in early axotomy-induced DRG neuron cell death and then this pathway is instead by other pathways. The Db/Db project was used to investigate the role of hyperglycemia on DRG neuron numbers. By counting DRG neuron numbers from typeâ…¡transgeneic mice and control group, we found that long hyperglycemic microenviroment around DRG could induce DRG neuron cell loss up to 30% and no further cell death occurred after 1 week of axotomy. The medium size DRG neurons showed clear susceptivity to hyperglycemia-induced DRG neuron cell death.
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